화학공학소재연구정보센터
검색결과 : 25건
No. Article
1 A new vector coupling ligation-independent cloning with sortase a fusion for efficient cloning and one-step purification of tag-free recombinant proteins
Jia XY, Crawford T, Zhang AH, Mobli M
Protein Expression and Purification, 161, 1, 2019
2 A universal mini-vector and an annealing of PCR products (APP)-based cloning strategy for convenient molecular biological manipulations
Liu X, Li TP, Hart DJ, Gao S, Wang HL, Gao HR, Xu SM, Zhang YF, Liu YF, An YF
Biochemical and Biophysical Research Communications, 497(4), 978, 2018
3 Cloning, expression and purification of kinase domains of cacao PR-1 receptor-like kinases
Tosarini TR, Ramos PZ, Profeta GS, Baroni RM, Massirer KB, Counago RM, Mondego JMC
Protein Expression and Purification, 146, 78, 2018
4 High-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC)
Camilo CM, Polikarpov I
Protein Expression and Purification, 99, 35, 2014
5 Ligase-Independent Cloning of Amylase Gene from a Local Bacillus subtilis Isolate and Biochemical Characterization of the Purified Enzyme
Ozturk MT, Akbulut N, Ozturk SI, Gumusel F
Applied Biochemistry and Biotechnology, 171(2), 263, 2013
6 Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification
Warren TD, Coolbaugh MJ, Wood DW
Protein Expression and Purification, 91(2), 169, 2013
7 Simplifying protein expression with ligation-free, traceless and tag-switching plasmids
Durani V, Sullivan BJ, Magliery TJ
Protein Expression and Purification, 85(1), 9, 2012
8 Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases
Luna-Vargas MPA, Christodoulou E, Alfieri A, van Dijk WJ, Stadnik M, Hibbert RG, Sahtoe DD, Clerici M, De Marco V, Littler D, Celie PHN, Sixma TK, Perrakis A
Journal of Structural Biology, 175(2), 113, 2011
9 Transfer-PCR (TPCR): A highway for DNA cloning and protein engineering
Erijman A, Dantes A, Bernheim R, Shifman JM, Peleg Y
Journal of Structural Biology, 175(2), 171, 2011
10 The pURI family of expression vectors: A versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins
Curiel JA, de las Rivas B, Mancheno JM, Munoz R
Protein Expression and Purification, 76(1), 44, 2011