Protein Expression and Purification

Protein Expression and Purification, Vol.46, No.2 Entire volume, number list

ISSN: 1046-5928 (Print)

In this Issue (42 articles)

173 - 178	High-throughput protein purification using an automated set-up for high-yield affinity chromatography Steen J, Uhlen M, Hober S, Ottosson J
179 - 188	Production and purification of self-assembling peptides in Ralstonia eutropha Reed DC, Barnard GC, Anderson EB, Klein LT, Gerngross TU
189 - 195	Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis Phan TTP, Nguyen HD, Schumann W
196 - 203	Cloning, expression, and purification of recombinant bovine rotavirus hemagglutinin, VP8*, in Escherichia coli Favacho ARM, Kurtenbach E, Sardi SI, Gouvea VS
204 - 211	Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae Niebauer RT, Robinson AS
212 - 220	Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase Chang WN, Tsai JN, Chen BH, Fu TF
221 - 232	Alkaline phytase from Lilium longiflorum: Purification and structural characterization Garchow BG, Jog SP, Mehta BD, Monosso JM, Murthy PPN
233 - 239	Overexpression and purification of scytovirin, a potent, novel anti-HIV protein from the cultured cyanobacterium Scytonema varium Xiong CY, O'Keefe BR, Botos I, Wlodawer A, McMahon JB
240 - 247	A rapid ATP affinity-based purification. for the human non-receptor tyrosine kinase c-Src Jeansonne DP, Bordes TJ, Bennett CA, Kothandaraman G, Bush JG, Vaccaro JA
248 - 255	Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization Inouye K, Minoda M, Takita T, Sakurama H, Hashida Y, Kusano M, Yasukawa K
256 - 267	Expression, purification, and characterization of Clostridium botulinum type B light chain Gilsdorf J, Gul N, Smith LA
268 - 273	Intracellular production of a soluble and functional monomeric streptavidin in Escherichia coli and its application for affinity purification of biotinylated proteins Wu SC, Wong SL
274 - 284	Extracellular production of Streptomyces lividans acetyl xylan esterase A in Escherichia coli for rapid detection of activity Nisole A, Lussier FX, Morley KL, Shareck F, Kazlauskas RJ, Dupont C, Pelletier JN
285 - 293	Expression and purification of human placenta lactogen in Escherichia coli Lan PC, Tseng CF, Lin MC, Chang CA
294 - 298	Overexpression and purification of tagged Escherichia coli proteins using a chromosomal knock-in strategy Jain C
299 - 308	An efficient method to express and refold a truncated human procaspase-9: A caspase with activity toward Glu-X bonds Sadhukhan R, Leone JW, Lull J, Wang ZG, Kletzien RF, Heinrikson RL, Tomasselli AG
309 - 315	Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli Olchowy J, Kur K, Sachadyn P, Milewski S
316 - 320	A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens Liu QF, Kirchhoff JR, Faehnle CR, Viola RE, Hudson RA
321 - 331	Production of a truncated soluble human semicarbazide-sensitive amine oxidase mediated by a GST-fusion protein secreted from HEK293 cells Ohman J, Jakobsson E, Kdllstrom U, Elmblad A, Ansari A, Kalderen C, Robertson E, Danielsson E, Gustavsson AL, Varadi A, Ekbloin J, Holmgren E, Doverskog M, Abrahmsen L, Nilsson J
332 - 336	Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin Yasukawa K, Kusano M, Nakamura K, Inouye K
337 - 342	High yield and secretion of recombinant human apolipoprotein AI in Pichia pastoris Feng MQ, Cai QS, Song DX, Dong JB, Zhou P
343 - 347	Improved purification protocol for wild-type and mutant human foamy virus proteases Boross N, Tozser J, Bagossi N
348 - 357	An efficient mammalian cell-free translation system supplemented with translation factors Mikami S, Masutani M, Sonenberg N, Yokoyama S, Imataka H
358 - 366	Cloning, bacterial expression, purification and structural characterization of N-terminal-repetitive domain of gamma-gliadin Benitez-Cardoza CG, Rogniaux H, Popineau Y, Gueguen J
367 - 373	Expression of bioactive human M-CSF soluble receptor in transgenic tobacco plants Zheng GG, Yang YH, Rao Q, Lin YM, Zhang B, Wu KF
374 - 378	Expression, purification, and renaturation of bone morphogenetic protein-2 from Escherichia coli Long SN, Truong L, Bennett K, Phillips A, Wong-Staal F, Ma HW
379 - 389	Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1 Nurmemmedov E, Thunnissen M
390 - 400	Synthesis of chicken major histo compatibility complex class II oligomers using a baculovirus expression system Niemiec PK, Read LR, Sharif S
401 - 405	Improvement in the expression of CYP2B6 by co-expression with molecular chaperones GroES/EL in Escherichia coli Mitsuda M, Iwasaki M
406 - 413	Structural characterization and binding properties of the hemopexin-like domain of the matrixmetalloproteinase-19 Mysliwy J, Dingley AJ, Sedlacek R, Grotzinger J
414 - 420	Identification and characterization of a constitutively T-loop phosphorylated and active recombinant S6K1: Expression, purification, and enzymatic studies in a high capacity non-radioactive TR-FRET Lance assay Zhang WG, Shor B, Yu K
421 - 428	Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate Kanwar SS, Ghazi IA, Chimni SS, Joshi GK, Rao GV, Kaushal RK, Gupta R, Punj V
429 - 437	Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: Purification and characterization Fonseca IO, Magalhaes MLB, Oliveira JS, Silva RG, Mendes MA, Palma MS, Santos DS, Basso LA
438 - 445	Combined hydrophobic-metal binding fusion tags for applications in aqueous two-phase partitioning Bernaudat F, Bulow L
446 - 455	Preparation of isotopically labeled spinach acyl-acyl carrier protein for NMR structural studies Zornetzer GA, White RD, Markley JL, Fox BG
456 - 467	Expression of the human activin type I and II receptor extracellular domains in Pichia pastoris Daly R, Hearn MTW
468 - 474	Production of active recombinant mitogen-activated protein kinases through transient transfection of 293T cells Zhao Q, Chen PL, Manson ME, Liu YS
475 - 482	Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources Lovingshimer MR, Siegele D, Reinhart GD
483 - 488	Single step protocol to purify recombinant proteins with low endotoxin contents Reichelt P, Schwarz C, Donzeau M
489 - 494	Prion protein from Xenopus laevis: Overexpression in Escherichia coli of the His-tagged protein and production of polyclonal antibodies Pagliato L, Negri A, Nonnis S, Taverna F, Sangiorgio L, Ronchi S, Tedeschi G
495 - 502	Expression, purification, and in vitro refolding of a humanized single-chain Fv antibody against human CTLA4 (CD152) Chen LH, Huang QA, Wan L, Zeng LY, Li SF, Li YP, Lu XF, Cheng JQ
503 - 509	Expression and characterization of human vascular endothelial growth factor (VEGF(165)) in insect cells Lee GY, Jung WW, Kang CS, Bang IS