Manganese (II) forms a yellow; coloured complex with a reagent picolinaldehyde nicotinoylhydrazone (PANH), which can be quantitatively extracted into chloroform in the pH range 8.7-10.5. Beer's law was obeyed over the concentration range of 0.02-1.5 ppm with absorption maxima at 385 nm. The molar absorptivity and Sandell's sensitivity of the extracted species was found to be 4.606 x 10(4) L mol(-1) cm(-1) and 1.19 ng cm(-2) respectively, The nature of the complex was determined by different methods and it was found to be 1:2 (Metal: Ligand). Interference due to diverse ions has been studied. The proposed method is simple, rapid, accurate, sensitive and has been applied for separation and determination of Mn(II) in synthetic mixtures and real samples.