Galactose oxidase has been immobilised on a solid support developed by modification and activation of surface of Ocimum sanctum seeds. The conversions of xylitol to L-xylose; galactitol to L-galactose and D-sorbitol to L-glucose using the above immobilised enzyme preparation have been shown. Galactose oxidase co-immobilised with catalase increases the conversion yields. Conversion of D-sorbitol to L-glucose using the immobilised galactose oxidase in a mini flow bed reactor has been demonstrated.