The thin layer chromatographic behaviour of sonic synthetic dyes has been studied on egg shell layers in various organic solvents and inorganic salt solutions. The mobility of the dyes was found to depend on the polarity of the solvents. Ketones gave compact spots of dyes. A large number of difficult separation of analytical interest have been achieved on the basis of Rr-RL value. The results are compared with the chromatographic behaviour of phenols to establish the separation potential of the egg shell layer in pollution studies.