The thin layer chromatography of metal ion has been performed in twelve solvent system's on egg shell layers. The separation of Mo6+ and W6+ from Ti4+, VO2+, Fe2+, Fe3+ , UO22+ , Ta5+ and Nb5+; Tl+ from Hg2+ and Pb2+; Hg2+ from Hg+ have been achieved on egg shell layers in distilled water, 0.1 M calcium nitrate and 0.1 M potassium thiocyanate, respectively. The separation on egg shell layers follow the ion exchange precipitation mechanism as evidenced by the X-ray analysis results and the solubility data of various carbonates.