A practicable, cost-effective and eco-friendly protocol of bagasse preservation for six months has been evolved. It envisages exposure of 4 ppm ozone once per month on 200 kg bagasse covered with polythene sheet. it afforded substantial (87%) reduction of bacterial, fungal and yeast count, concomitantly retaining initial properties of fiber, largely arresting degradation of cellulose and hemi-cellulose content and thereby maximally retaining initial brightness (reduction by meager 5.7%).