Yeast cells of Saccharomyces cerevisiae were found to accumulate (51)Cr(III) radioisotope at basic pH in trace level. There was no uptake of Cr(VI) at the same pH (similar to 10.5). An assay of the products of cell lysis reveals that Cr(III) first gets absorbed at the cell wall and then slowly enters the cytoplasm which increases with time, attains a maximum value and then release the metal ion from cytoplasm. The behavior and uptake kinetics of (51)Cr(III) or (51)Cr(VI) were studied using gamma-spectrometry.