The frictional forces between pressure sensitive adhesives (PSAs) and a probe tip were measured with a scanning probe microscopy (SPM). A peak appeared in the scanning rate-frictional force curve shifted to a lower scanning rate with decreasing temperature. In the case of the miscible system of isoprene matrix of SIS base polymer, the tendency of a peak to shift to a lower scanning rate was observed with increasing tackifier content; however, in the case of the immiscible system of styrene domain of SIS base polymer, no remarkable shift was observed. The frictional force is influenced by viscoelastic properties of the PSA which systematically changed with miscibility. In this study, it is aimed to clarify the correlation between the observation of phase structure and the behavior of surface rheology by using two kinds of tackifiers that have different miscibility with the polyisoprene phase or the polystyrene phase of SIS triblock copolymer.