The solubility of pyoluteorin (Plt) in water, dichloromethane, chloroform, and carbon tetrachloride was determined using an analytical method over the temperature range of (278.2 to 333.2) K.. The solubility of Plt in chloroform is much higher than that in water, dichloromethane, and carbon tetrachloride. The solubility of Plt increases with increasing temperature in the four solvents, and all measurements were correlated with the Apelblat equation.