A microchannel for the formation of droplets was fabricated on an acrylic resin plate by mechanical machining. The flow behavior in a droplet formed through a microchannel was investigated. A computational fluid dynamics (CFD) simulation was conducted to investigate the fluid flow during the formation of a hexane droplet in water from a micronozzle with diameter of 100 mum. As a result, the fluid in the droplet was found to recirculate at a high rate due to the effect of contraction flow formed by an expanding droplet in microspace. An experimental study was conducted to validate the simulation results. A continuous n-hexane phase was introduced into the microchannel. Water containing a small number of fluorescent particles was fed from a branch channel. The rotating trajectories of fluorescent particles in the water droplet were then observed.