A novel ion-exchange moving bed has been developed which uses resin-liquid slurry tanks, thereby allowing fermentation broth to be fed directly to the resin process without pretreatment. A previous study using L-Lysine fermentation broth demonstrated that clogging by bacteria did not occur, but such problems as poor performance in the elution process and instability of the equipment remained. To overcome these problems, the experimental apparatus was improved by using a new type of resin separator. Stable operation was achieved, and the production Of L-Lysine reached 15.4 ton in 12 days. The elution process was especially improved compared to the previous work. The amount of the eluent could be reduced and the concentration Of L-Lysine in the eluate increased. The novel method also produced a smaller amount of waste water than the conventional fluidized bed. In addition, a numerical simulation model was constructed in order to optimize the operation. The predictions from the simulation agreed well with the experimental data.