The adsorption experiment of divalent transition metals, such as cobalt, nickel, copper and zinc on porous glass from ethylenediamine aqueous solution was carried out at 298 K. The comparison between the experimental values of amount of metal adsorbed and the formation curves of ethylenediamine-metal complex ions suggested that adsorption mechanism was coordination reaction, because adsorption of metal was accelerated with formation of ethylenediamine complex ion in the case of nickel, copper and zinc. The contribution of ion-exchange to adsorbed amount was negligibly small comparing with coordination reaction. It seemed that trivalent cobalt ion was formed by the oxidation in exchanging mother solution, then it was adsorbed by ion exchange for adsorption of cobalt. The separation experiment of the metals mentioned above was carried out by the porous glass packed column using ethylenediamine as an eluent at 298 K. The retention times of these metals were increased with amount of metal adsorbed in all cases. Cobalt, nickel and copper were eluted by this order and almost completely separated in the range of ethylenediamine concentration less than 0.2 mol/dm(3), when the eluent was used at pH 6.0. In this case zinc was not isocratically eluted, because zinc was adsorbed much than three other metals.