Enzymatic and ultrasonic degradations of the carboxymethyl derivative of the beta-1,3-D-glucan, isolated from the cell walls of baker’s yeast Saccharomyces cerevisiae, are described. The samples obtained upon partial depolymerization of the two carboxymethylated glucans with the substitution degree of 0.56 and 0.91 were characterized by the distributions of their molecular weights and C-13-NMR spectra.