N-Alkenylammonium chlorides were synthesized from diethylamine, 4-chloropent-2-ene, and a mixture of 2-methyl-3-chlorobut-1-ene and 3-methyl-3-chlorobut-1-ene. The reaction conditions were optimized. The structure of the products was proved and the main physicochemical characteristics of the synthesized compounds were determined. The synthesized quaternary ammonium salts were tested for bactericidal activity towards corrosion-active microorganisms.