This study introduces measurements of light scattering intensities individually produced from polystyrene microspheres illuminated by focused Gaussian beams in a flow environment. These light scattering signatures are correlated to the particle size. The Fourier domain information of the light scattering signatures are interpreted and translated to recover the dimension of each particle. A hardware-software interface is designed to capture, analyze and interpret the collection of various scattering light intensities. A major contribution of this study is in consolidating the implementation of the Lorenz-Mie theory with the experimental results of collected light scatterings for the purpose of particle sizing through novel pattern analysis techniques. Ln so doing, a synergetic approach is considered in bringing together the hardware design with the software implementations for an integrated hardware-software interface.