Based on the statistical characteristics of the transmission fluctuations in the particle suspension, transmission fluctuation spectrometry with autocorrelation (TFSAC) is described theoretically, with the assumptions of geometric ray propagation and completely absorbent particles in the suspension. The experiments presented here are realized in a focused Gaussian beam with the TFS-AC technique. The acquisition of transmission fluctuation signals is achieved by using a high-resolution digital oscilloscope. The transition function of TFS-AC is obtained by varying the autocorrelation time. With a modified iterative Chahine inversion algorithm, solving a linear equation retrieves information on the particle size distribution and particle concentration. Some experimental results on spherical and non-spherical particles are presented and discussed. The experiments cover a particle size range from 1 mu m to 1000 mu m and a particle concentration of up to 12 %.