Segmented Polyurethanes were prepared by chain extending the prepolymers based on poly(tetramethylene oxide)glycol and tolylene diisocyanate using fluorescein. The polymers were characterized using FTNMR and FTIR. Thermal stability of the polymers was studied using a thermogravimetric analyzer, and the glass transition temperature of the polymers was determined using a differential scanning calorimeter. Tensile strength and percentage of elongation of these segmented polyurethanes were evaluated using a Universal Testing Machine.