Microbial fuel cell (MFC) consisting of 3 (anodic, cathodic and nourishment) chambers was inoculated with anaerobic fresh or adapted sludge and used for studying its properties. The catholyte consisted of K(3)Fe(CN)(6), KH(2)PO(4) and NaOH. The anolythe (nourishment) was a glucose soln. Electrochem. characterization of MFC was carried out by cyclic voltammetry, chronopotentiometry and spectroscopic electrochem. impedance measurements. The MFC accomplished max. values of voltage 721 mV and power d. 71,9.10(-2) W/m(2). The internal resistance of MFC was 3 Omega.