Lethal or harmful effects of X-rays and gamma rays are known to be reduced by prior administration of certain "radioprotector" chemicals. In this work, complex formation of polyacrylamide with bovine serum albumin (as a model protein) in the presence of divalent copper ions (Cu (2+)) was investigated with high-performance liquid chromatography (HPLC) before evaluating its possible use as a radioprotector. HPLC results determined the most suitable metal concentration and protein/polymer ratio for maximum complex formation between polymer and protein molecules.