Nonswellable and swellable poly(ethyleneglycol dimethacrylate)-based microbeads that could react directly with the biological molecules were produced by a suspension polymerization procedure. For this purpose, ethyleneglycol dimethacrylate (EGDMA) was copolymerized with glycidyl methacrylate (GMA) in an aqueous suspension medium. Benzoyl peroxide and poly(vinyl alcohol) were used as the initiator and the stabilizer, respectively. The copolymerization provided nonswellable, tranparent, and spherical copolymer microbeads in the size range of 100-300 mu m. On the other hand, swellable copolymer microbeads in the aqueous medium were obtained by using toluene as a diluent in the same copolymerization recipe. In a separate group of polymerizations, styrene (St) monomer was also included within the monomer phase to regulate the hydrophobicity of resulting microbeads. Nonswellable and swellable poly(EGDMA-St-CMA) microbeads were obtained by changing the type and concentration of the ingredients within the monomer phase. The effects of glycidyl methacrylate, styrene, and toluene concentrations on the microbead yield, the average size, and the swellability of microbeads were investigated. In the second part of the study, the interaction of produced microbeads with a selected enzyme (i.e., chymotrypsin) was investigated. The most stable chymotrypsin immobilization was achieved with the swellable poly(EGDMA)-based microbeads including styrene.