Using micro-contact printing (mu CP) method, lipid membranes were deposited on the surface of micro-patterned self-assembled monolayers. Height and phase mode atomic force microscopy (AFM) images showed that the resulting deposited films were flat and that a micro-patterned composite bilayer system was constructed. It was also shown that the use of crystalline phase lipid membrane is effective for the preparation of the micro-patterned composite bilayer system of membranes with flat surface. (C) 2008 Elsevier B.V. All rights reserved.