We report results of studies relating to preparation and characterization of DNA electrodes based on the chitosan-MWCNT/ITO nanobiocomposite platform for electrochemical detection of gonorrhoea, the most common bacterial sexually transmitted disease. (STD). This biosensing electrode has been characterized using FT-IR. SEM, cyclic voltammetry and differential pulse voltammetry, etc. The sensing characteristics have been investigated in phosphate buffer saline using methylene blue as the hybridization indicator. This DNA biosensor has a response time of 60 s and is found to be stable for about 4 months when stored at 4 degrees C The results of DPV studies reveal that this bioelectrode can detect complementary DNA concentration in a wide range of 1 x 10(-6) M to 1 x 10(-17) M with a detection limit of 1 x 10(-16) M. (C) 2010 Elsevier B.V. All rights reserved.