Use of biologically designed gold nanowire for biosensor application

Sung-Hee Shin; Gha-Young Kim; Joonmok Shim; Jungok Kim; Hor-Gil Hur; Don-Jung Lee; Jong-In Song; Seung-Hyeon Moon

Korean Journal of Chemical Engineering, Vol.29, No.12, 1666-1669, December, 2012

DOI10.1007/s11814-012-0140-y Export Citation

Use of biologically designed gold nanowire for biosensor application

Sung-Hee Shin, Gha-Young Kim¹, Joonmok Shim², Jungok Kim, Hor-Gil Hur , Don-Jung Lee³, Jong-In Song³, and Seung-Hyeon Moon^†

School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 123, Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Korea
¹Department of Nuclear Fuel Cycle Technology Development, Korea Atomic Energy Research Institute, Yuseong-gu, Daejeon 305-701, Korea
²Energy Storage Center, Korea Institute of Energy Research (KIER), 152, Gajeong-ro, Yuseong-gu, Daejeon 305-343, Korea
³Department of Nanobio Materials and Electronics, Gwangju Institute of Science and Technology (GIST), 123, Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Korea

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A highly sensitive tyrosinase (TYR)-based amperometric biosensor is prepared using biologically designed gold nanowires (AuNWs) for pesticide detection. The AuNWs were synthesized by dodecapeptide Midas-11 and were modified with the formation of self-assembled monolayer (SAM), followed by covalent binding with TYR. The prepared TYR-AuNWs-SPCE (screen printed carbon electrode) was compared with bare, AuNWs-, modified-AuNWs-SPCE by the measurement of cyclic voltammetry. The quantitative relationship between the inhibition percentage and the pesticide concentration at the TYR-AuNWs-SPCE was obtained by measuring the current response in various concentrations of pesticides. The reasonable detection range of parathion was determined to be 0.1 ppt through 10 ppb (R2=0.990) with 0.087 ppt of detection limits. The higher sensitivity and wider detection range of the TYR-based biosensor was achieved by the use of biologically synthesized AuNWs.

Keywords:Tyrosinase;Gold Nanowire;Peptide;Amperometric Biosensor;Pesticide

[References]

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de Albuquerque YDT, Ferreira LF, Anal. Chim. Acta., 596, 210 (2007)
Lee HS, Kim YA, Cho YA, Lee YT, Chemosphere., 46, 571 (2002)
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Kia M, Islamnezhad A, Shariati S, Biparva P, Korean J. Chem. Eng., 28(10), 2064 (2011)
Kim GY, Shim J, Kang MS, Moon SH, J. Hazard. Mater., 156(1-3), 141 (2008)
Park BW, Kim DS, Yoon DY, Korean J. Chem. Eng., 28(1), 64 (2011)
Liu GD, Wang J, Barry , Petersen C, Timchalk C, Gassman PL, Lin YH, Chem. Eur. J., 14, 9951 (2008)
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Cherevko S, Chung CH, Sensors and Actuators, B: Chemical., 142, 216 (2009)
Kim J, Rheem Y, Yoo B, Chong Y, Bozhilov KN, Kim D, Sadowsky MJ, Hur HG, Myung NV, Acta Biomater., 6, 2681 (2010)
Kim GY, Shim J, Kang MS, Moon SH, J. Environ. Monit., 10, 632 (2008)
Gorelik LY, Voinova MV, Biosens. Bioelectron., 22, 405 (2006)
Shim J, Woo JJ, Moon SH, Kim GY, J. Membr. Sci., 330(1-2), 341 (2009)
Lin YH, Lu F, Wang J, Electroanalysis., 16, 145 (2004)
Du D, Huang X, Cai H, Zhang AD, Biosens. Bioelectron., 23, 285 (2007)
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Viswanathan S, Radecka H, Radecki J, Biosens. Bioelectron., 24, 2772 (2009)

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[2] Boye B, Brillas E, Marselli B, Michaud PA, Comninellis C, Farnia G, Sandona G, Electrochim. Acta, 51(14), 2872 (2006)

[3] Cha DK, Sarr D, Chiu PC, Kim DW, Water Environ. Res., 70, 705 (1998)

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[5] de Albuquerque YDT, Ferreira LF, Anal. Chim. Acta., 596, 210 (2007)

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[12] Liu GD, Wang J, Barry , Petersen C, Timchalk C, Gassman PL, Lin YH, Chem. Eur. J., 14, 9951 (2008)

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