Isolation and characterization of autoflocculating mutants of cyanobacterium Arthrospira platensis

Young-Hwa Kim; Hyun-Jin Park; Sang-Hoon Lee; Jae-Hwa Lee

Korean Journal of Chemical Engineering, Vol.30, No.2, 413-416, February, 2013

DOI10.1007/s11814-012-0146-5 Export Citation

Isolation and characterization of autoflocculating mutants of cyanobacterium Arthrospira platensis

Young-Hwa Kim¹, Hyun-Jin Park², Sang-Hoon Lee³, and Jae-Hwa Lee^{1, 2, †}

¹Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University, Busan 617-736, Korea
²Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea
³Korea Food Research Institute, Bundang-gu, Sungnam-si, Gyeonggi-do 463-746, Korea

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Harvesting microalgae is a major concern for mass culture in industry. Flocculation is an easy and effective way to harvest microalgae. However, flocculation using chemical flocculants is not feasible for scaling-up due to their toxicity. As an alternative technique, mutation breeding of autoflocculating microalgae strain has been reported in this study. We characterized autoflocculating mutants of Arthrospira platensis (A. platensis) by ethyl methane sulfonate (EMS). The cells of mutants were aggregated during the culture and dry cell weight increased 1.2- to 1.8-fold compared to the wild type. Autoflocculation was induced highly at an optimal pH level of 9 and the flocculation efficiency reached almost 90%. Mutants showed higher flocculation efficiency irrespective of the addition of chemical flocculants. Thus, it is definitely useful to harvest microalgae using autoflocculating mutants in large-scale culture without any drawbacks of harvested algal biomass.

Keywords:Microalgae;Arthrospira platensis;Autoflocculation;Ethyl Methane Sulfonate;Mutation

[References]

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Shimamatsu H, Hydrobilogia., 521, 39 (2004)
Harith ZT, Yusoff FM, Mohamed MS, Shariff M, Din M, Ariff AB, African J. Biotechnol., 8, 5971 (2009)
Uduman N, Qi Y, Danquah MK, Forde GM, Hoadley A, J. Renew. Sustain. Energy., 2, 012701 (2010)
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Sandbank E, Hepher B, Ergeb. Limnol., 11, 108 (1978)
Sukenik A, Bilanovic D, Shelef G, Biomass., 15, 187 (1988)
Kwon GS, Moon SH, Hong SD, Lee HM, Kim HS, Oh HM, Yoon BD, Biotechnol. Lett., 18(12), 1459 (1996)
Lee SJ, Lee YJ, Nam SH, Korean J. Chem. Eng., 25(3), 505 (2008)
Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgnug JH, Posten C, Kruse O, Hankamer B, Bioenerg. Res., 1, 20 (2008)
Lee SJ, Kim SB, Kim JE, Kwon GS, Yoon BD, Oh HM, Lett. Appl. Microbiol., 27, 14 (1998)
Salim S, Bosma R, Vermue MH, Wijffels RH, J. Appl. Phycol., 23, 849 (2011)
Cao Y, Yao J, Li J, Chen X, Wu J, Elecronic J. Biotechnol., 13, 1 (2010)
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Ogbonda KH, Aminigo RE, Abu GO, Bioresour. Technol., 98(11), 2207 (2007)
Pandy JP, Pathak N, Tiwari A, J. Algal Biomass Utln., 1, 93 (2010)
Oliver RL, Ganf GG, Ed. Whitton BA, Potts, Dordrecht M, The Nethelands, Kluwer Academic Publishers, 149 (2000)
Yoo C, Kim CJ, Choi GG, Ahn CY, Choi JS, Oh HM, Kor. J. Microbiol., 45, 268 (2009)
Grima EM, Belarbi EH, Fernandez A, Medina AR, Christi Y, Biotechnol. Adv., 20, 291 (2003)
Wu JY, Ye HF, Process Biochem., 42, 114 (2007)
Kim SJ, Choi A, Ahn CY, Park CS, Park YH, Oh HM, Lett. Appl. Microbiol., 40, 190 (2005)

[Referenced By]

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[1] Chen F, Zhang YM, Enzyme Microb. Technol., 20(3), 221 (1997)

[2] Shimamatsu H, Hydrobilogia., 521, 39 (2004)

[3] Harith ZT, Yusoff FM, Mohamed MS, Shariff M, Din M, Ariff AB, African J. Biotechnol., 8, 5971 (2009)

[4] Uduman N, Qi Y, Danquah MK, Forde GM, Hoadley A, J. Renew. Sustain. Energy., 2, 012701 (2010)

[5] Wijffels RH, Barbosa MJ, Science, 329(5993), 796 (2010)

[6] Bilanovic D, Shelef G, Sukenik A, Biomass., 17, 65 (1988)

[7] Sandbank E, Hepher B, Ergeb. Limnol., 11, 108 (1978)

[8] Sukenik A, Bilanovic D, Shelef G, Biomass., 15, 187 (1988)

[9] Kwon GS, Moon SH, Hong SD, Lee HM, Kim HS, Oh HM, Yoon BD, Biotechnol. Lett., 18(12), 1459 (1996)

[10] Lee SJ, Lee YJ, Nam SH, Korean J. Chem. Eng., 25(3), 505 (2008)

[11] Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgnug JH, Posten C, Kruse O, Hankamer B, Bioenerg. Res., 1, 20 (2008)

[12] Lee SJ, Kim SB, Kim JE, Kwon GS, Yoon BD, Oh HM, Lett. Appl. Microbiol., 27, 14 (1998)

[13] Salim S, Bosma R, Vermue MH, Wijffels RH, J. Appl. Phycol., 23, 849 (2011)

[14] Cao Y, Yao J, Li J, Chen X, Wu J, Elecronic J. Biotechnol., 13, 1 (2010)

[15] Queener SW, Lively DH, In: Demain AL, Solomon NA, Ed., American Soc. Microbiol., Washington, DC, 155 (1986)

[16] Knuckey RM, Brown MR, Robert R, Frampton DMF, Aquacult. Eng., 35, 300 (2006)

[17] Torzillo G, Scoma A, Faraloni C, Ena A, Johanningmeier U, Int. J. Hydrog. Energy., 34, 4529 (2009)

[18] Polle JEW, Kanakagiri S, Jin E, Masuda T, Melis A, Int. J.Hydrog. Energy., 27, 1257 (2002)

[19] Divakaran R, Pillai VNS, J. Appl. Phycol., 14, 419 (2002)

[20] Ogbonda KH, Aminigo RE, Abu GO, Bioresour. Technol., 98(11), 2207 (2007)

[21] Pandy JP, Pathak N, Tiwari A, J. Algal Biomass Utln., 1, 93 (2010)

[22] Oliver RL, Ganf GG, Ed. Whitton BA, Potts, Dordrecht M, The Nethelands, Kluwer Academic Publishers, 149 (2000)

[23] Yoo C, Kim CJ, Choi GG, Ahn CY, Choi JS, Oh HM, Kor. J. Microbiol., 45, 268 (2009)

[24] Grima EM, Belarbi EH, Fernandez A, Medina AR, Christi Y, Biotechnol. Adv., 20, 291 (2003)

[25] Wu JY, Ye HF, Process Biochem., 42, 114 (2007)

[26] Kim SJ, Choi A, Ahn CY, Park CS, Park YH, Oh HM, Lett. Appl. Microbiol., 40, 190 (2005)