Essential oil was extracted from lavender using supercritical carbon dioxide by means of a newly developed periodic static-dynamic (PSD) procedure and the conventional semicontinuous (SC) technique. Applying GC-FID analysis in conjunction with Box-Behnken design, an optimum overall extraction yield (94.4?%) was obtained via PSD in contrast to 90?% for the SC method. The results indicate that supercritical fluid extraction is a viable technique for separation of constituents such as linalyl acetate, linalool, fenchone, and camphor for pharmaceutical and medicinal applications. Furthermore, a substantial reduction of energy consumption and solvent consumption is achieved with the developed PSD process compared to the conventional SC method.