The aim of the present study was to synthesize and examine the biological properties of soft gemini bis-quaternary surfactants, derivatives of bis-alanine esters. As a model system for the investigation of the activity of gemini surfactants Candida albicans strains with deletions of gene encoded ABC transporters were used. TMPAL-12Br was more active than TMPAL-10Br in dislodging of the C. albicans biofilms. The results of the present study may prove useful in antifungal applications.