In this work, the effects of stirring and bubbling methods on hydrate-based carbon dioxide (CO2) separation from integrated gasification combined cycle (IGCC) synthesis gas are compared. Then, an integrated process of bubbling in conjunction with temperature fluctuation is proposed and adopted in the experiments, which are conducted in bench and scaled-up equipment. The experimental results show that the bubbling method has a similar positive effect on the CO2 separation as the stirring method. The optimal volume ratio of tetra-n-butylammonium bromide (TBAB) solution to the reactor shifts to 0.75 after the volume of the reactor is enlarged 100-fold, and at that ratio, the total 15.3 mol of gas is consumed and the mole concentration of CO2 in the gas phase reduces from 40.0 to 13.2%. The results indicate that the integrated process and scaled-up equipment are feasible for hydrate-based CO2 separation.