Aims To prepare concentrated formulation of Trichoderma harzianum MTCC-3841 (NBRI-1055) with high colony forming units (CFU), long shelf life and efficient in root colonization by a simple scrapping method. Methods and Results NBRI-1055 spores scrapped from potato dextrose agar plates were used to prepare a concentrated formulation after optimizing carrier material, moisture content and spore harvest time. The process provides an advantage of maintaining optimum moisture level by the addition of water rather than dehydration. The formulation had an initial 1112 log10 CFU g-1. Its concentrated form reduces its application amount by 100 times (10 g 100 kg-1 seed) and provides 34 log10 CFU seed-1. Shelf life of the product was experimentally determined at 30 and 40 degrees C and predicted at other temperatures following Arrhenius equation. The concentrated formulation as compared to similar products provides an extra advantage of smaller packaging for storage and transportation, cutting down product cost. Seed application of the formulation recorded significant increase in plant growth promotion. Conclusions Stable and effective formulation of Trichoderma harzianum NBRI-1055 was obtained by a simple scrapping method. Significance and Impact of the Study A new method for the production of concentrated, stable, effective and cost efficient formulation of T. harzianum has been validated for seed application.