Permeation of molecules through the membranes plays an important role in immobilized cell systems. Permeation of molecules libe glucose and bovine albumin serum was studied through chitosan, polyvinyl alcohol, and polyvinyl acetate membranes using a flow cell. made of two detachable compartments. Permeation of molecules through chitosan, polyvinyl alcohol, and polyvinyl acetate membranes increases with decrease in hydrophobic characteristics of the membrane. Permeation of molecules also show its dependency on the molecular weight of the solute. Distribution coefficient of glucose and bovine serum albumin in these polymeric membranes measured by equilibration technique indicates that permeation of molecules through these membranes follows pore type mechanism.