We performed high resolution marking experiments using seawater with elevated Sr concentration to investigate the timing and ultrastructure of skeletal deposition by massive Porites australiensis corals. Corals were cultured in seawater enriched with Sr during day-time only, night-time only or for one full-day. Cross sections of skeletal material were prepared and the Sr incorporated into the newly deposited skeleton analyzed by electron probe microanalysis. These regions of Sr incorporation were then correlated with skeletal ultrastructure. Massive Pontes coral skeletons are composed of two types of microstructural elements - the "centers of calcification" and the surrounding fibrous structural region. Within the fibrous structural region, alternative patterns of etch-sensitive growth lines and an etch-resistant fibrous layer were observed. In the full-day samples, high-Sr bands extended across both growth lines and fibrous layers. In day-time samples, high-Sr regions corresponded to the fibrous layer, while in the night-time samples high-Sr regions were associated with an outermost growth line. These distinct growth patterns suggest a daily growth pattern associated with the fibrous region of massive P. australiensis corals, where a pair of narrow growth lines and a larger fibrous layer is seen as a daily growth region. (C) 2012 Elsevier Inc. All rights reserved.