Korean Chemical Engineering Research, Vol.51, No.1, 42-46, February, 2013
미역으로부터 후코산틴 추출 및 후코산틴 안정성
Extraction of Fucoxanthin from Undaria pinnatifida and Stability of Fucoxanthin
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초록
미역에서 후코산틴을 추출하는 공정과 후코산틴의 안정성에 대해 연구하였다. 본 연구에서는 후코산틴 추출물을 기능성 식품에 이용하기 위해 용매를 에탄올을 사용했다. 에탄올 농도가 80%일 때 후코산틴 추출 농도가 최고였다. 50 ℃까지 추출온도가 상승할 때 추출수율이 증가하였다. 원료를 5분간 물세척하여 후코산틴 중 염분농도를 약 94% 감소시켰다. 후코산틴이 70 ℃에서 1일 저장 동안 30% 감소해, 70 ℃ 이상의 온도에서 열에 약함을 나타냈다. 그리고 빛 안정성 실험결과 후코산틴은 빛에도 매우 약함을 보였다.
Extraction process of fucoxanthin from Undaria pinnatifida and stability of fucoxanthin was studied. In this study, to utilized extracted components as a functional food material, ethanol was used as a solvent. The maximum concentration of fucoxanthin was obtained when 80% ethanol solvent was used. The extraction yield of fucoxanthin increased as extraction temperature raised to 50 ℃. Water washing of raw material for 5 minutes reduced the salt content about 94%. From the experiment that fucoxanthin content reduced by 30% for 1 day storage at 70 ℃, it was demonstrated that fucoxanthin was thermal-unstable above 70 ℃. And experimental result of light- stability showed that fucoxanthin was very unstable with light.
- Nina G, Sergiu A, Kathi G, Anja B, Claudia B, Josef W, Biochimica et Biophysica Acta., 1797, 543 (2010)
- Thomas V, Jan B, Wolfram W, Maria M, Claudia B, Biochimica et Biophysica Acta., 1787, 905 (2009)
- Lavanya P, Bruno R, Anja B, Claudia B, Biochimica et Biophysica Acta., 1797, 1647 (2010)
- Nina G, Julia H, Kathi G, Claudia B, Josef W, Chem. Phys., 373, 104 (2010)
- Liu CL, Liang AL, Hu ML, Toxicol. Vitro., 25, 1314 (2011)
- Heo SJ, Jeon YJ, J. Photochem. Photobiol. B-Biol., 95, 101 (2009)
- Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K, Biochim. Biophys. Acta., 1675, 113 (2004)
- Das SK, Hashimoto T, Shimizu K, Yoshida T, Sakai T, Sowa Y, Komoto A, Kanazawa K, Biochim. Biophys.Acta., 1762, 328 (2005)
- You SG, Yang C, Lee HY, Lee BY, Food Chem., 119, 554 (2010)
- Kim KN, Heo SJ, Kang SM, Ahn G, Jeon YJ, Toxicol. Vitro., 24(6), 1648 (2010)
- Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K, Biochimica et Biophysica Acta., 1675, 113 (2004)
- Satomi Y, Nishino H, Biochimica et Biophysica Acta., 1790, 260 (2009)
- Nakazawa Y, Sashima T, Hosokawa M, Miyashita K, Journal of Functional Foods., 1, 88 (2009)
- Swadesh KD, Hashimoto T, Kanazawa K, Biochimica et Biophysica Acta., 1780, 743 (2008)
- Bae JS, Lee JS, Kim YS, Sim WJ, Lee H, Chun JY, Park KP, Korean Chem. Eng. Res., 46(5), 886 (2008)
- Heo SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH, Affan A, Oh C, Jung WK, Jeon YJ, Food Chem. Toxicol., 48, 2045 (2010)
- Woo MN, Jeon SM, Kim HJ, Lee MK, Shin SK, Shin YC, Park YB, Choi MS, Chemico-Biological Interactions., 186, 316 (2010)
- Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu, F.,, Arch. Biochem. Biophys., 504(1), 17 (2010)
- Maeda H, Hosokawa M, Sashima T, Miyashita K, J. Agric. Food Chem., 55, 7701 (2007)
- Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K, Biochem. Biophys. Res. Commun., 332(2), 392 (2005)
- Wang WJ, Wang GC, Zhang M, Tseng CK, J. Integr. Plant Biol., 47, 1009 (2005)
- Kanazawa K, Ozaki Y, Hashimoto T, Das SK, Matsushita S, Hirano M, Okada T, Komoto A, Mori N, Nakatsuka M, Food Sci. Technol. Res., 14, 573 (2008)
- Rho MK, Uddin MS, Chun BS, Biotechnol. Bioprocess E., 13, 724 (2008)
- Shang YF, Kim SM, Lee WJ, Um BH, J. Biosci. Bioeng., 111(2), 237 (2011)
- Lee JY, Kim JH, Korean J. Chem. Eng., 28(7), 1561 (2011)