화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.5, 1105-1110, May, 2013
DOI10.1007/s11814-012-0219-5  Export Citation
Enhanced production of carboxymethylcellulase by Cellulophaga lytica LBH-14 in pilot-scale bioreactor under optimized conditions involved in dissolved oxygen
Wa Cao1, 2, Sang-Un Lee1, 2, Jianhong Li3, and Jin-Woo Lee2, 4, †
  • 1Department of Medical Bioscience, Graduate School of Dong-A University, Busan 604-714, Korea
  • 2BK21 Bio-Silver Program of Dong-A, Busan 604-714, Korea
  • 3College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hebei 430070, China
  • 4Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea
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The optimal conditions for the production of carboxymethylcellulase (CMCase) by Cellulophaga lytica LBH-14 at flask scale has been previously reported. In this study, we optimized the parameters involved in dissolved oxygen in 7 and 100 L bioreactors for pilot-scaled production of CMCase by C. lytica LBH-14. The optimal conditions of agitation speed and aeration rate for cell growth in 7 L bioreactors were 395 rpm and 0.98 vvm, whereas those for production of CMCase were 357 rpm and 0.55 vvm. The optimal inner pressures for cell growth and production of CMCase by C. lytica LBH-14 in 100 L bioreactors were 0.00 and 0.06MPa, respectively. The production of CMCase under an optimized inner pressure was 1.38 times higher than that without an inner pressure. The maximal production of CMCase by C. lytica under optimized conditions at pilot scale using rice bran and ammonium chloride was 153.6U/mL, which was 1.39 times higher than that at flask scale.
Keywords:Cellulophaga lytica;Carboxymethylcellulase;Marine Microorganism;Pilot-scaled Production;Response Surface Method
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