Biofilm-inactivating activity of silver nanoparticles: A comparison with silver ions

Hee-Jin Park; Soomin Park; Jinkyu Roh; Sujin Kim; Kyunghee Choi; Jongheop Yi; Younghun Kim; Jeyong Yoon

Journal of Industrial and Engineering Chemistry, Vol.19, No.2, 614-619, March, 2013

DOI10.1016/j.jiec.2012.09.013 Export Citation

Biofilm-inactivating activity of silver nanoparticles: A comparison with silver ions

Hee-Jin Park, Soomin Park, Jinkyu Roh¹, Sujin Kim², Kyunghee Choi², Jongheop Yi , Younghun Kim^{1, †}, and Jeyong Yoon^†

World Class University (WCU) Program of Chemical Convergence for Energy & Environment (C2E2), School of Chemical and Biological Engineering, College of Engineering, Institute of Chemical Process, Seoul National University (SNU), Daehak-dong, Gwanak-gu, Seoul 151-742, Republic of Korea
¹Department of Chemical Engineering, Kwangwoon University, 447-1 Wolgye-dong, Nowon-gu, Seoul 139-701, Republic of Korea
²National Institute of Environmental Research, Kyungseo-Dong, Seo-Gu, Incheon 404-708, Republic of Korea

E-mail:,

The antimicrobial activity of silver nanoparticles (AgNPs) against Pseudomonas aeruginosa PA01 planktonic and biofilm bacteria was examined; their activity was compared with that of silver ions. The inactivation of biofilms by AgNPs was greatly influenced by stirring, which caused an increased AgNP biosorption. Although the activity of AgNPs against planktonic cells was ca. 10% that of silver ions, their activity against biofilm cells was comparable to the silver ions’ activity at the same concentration after 90 min under stirring (ca. 3.5 log inactivation). AgNPs inactivated biofilms in a biosorption-dependent manner, whereas this was not the case for silver ions.

Keywords:Silver nanoparticle (AgNP);Biofilm;Pseudomonas aeruginosa;Silver ion;Biosorption

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Project on Emerging Nanotechnologies, Consumer Products Analysis (2009)
Sondi I, Salopek-Sondi B, J. Colloid Interface Sci., 275(1), 177 (2004)
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Dror-Ehre A, Adin A, Markovich G, Mamane H, Water Research., 44, 2601 (2010)
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[2] Dubas S, Kumlangdudsana P, Potiyaraj P, Colloids and Surface A., 289, 289 (2006)

[3] Benn T, Westerhoff P, Environmental Science and Technology., 42, 4133 (2008)

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[9] Lok C, Ho C, Chen R, He Q, Yu W, Sun H, Tam P, Chiu J, Che C, Journal of Proteome Research., 5, 916 (2006)

[10] Hwang E, Lee J, Chae Y, Kim Y, Kim B, Sang B, Gu M, Small., 4, 746 (2008)

[11] Rai M, Yadav A, Gade A, Biotechnology Advances., 27, 76 (2009)

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[13] Wigginton NS, Titta AD, Piccapietra F, Dobias J, Nesatyy VJ, Environmental Science and Technology., 44, 2163 (2010)

[14] Ivask A, Bondarenko O, Jepihhina N, Kahru A, Analytical and Bioanalytical Chemistry., 398, 701 (2010)

[15] John LP, Mark ER, Roger GF, Biofilm, Infection, and Antimicrobial Therapy, CRS Press, Boca Raton (2006)

[16] Mah T, O’Toole G, Trends in Microbiology., 9, 34 (2001)

[17] Nickel J, Ruseska I, Wright J, Costerton J, Antimicrobial Agents and Chemotherapy., 27, 619 (1985)

[18] Piriou P, Dukan S, Levi Y, Jarrige P, Water Science and Technology., 35, 283 (1997)

[19] Videla HA, International Biodeterioration and Biodegradation., 49, 259 (2002)

[20] Costerton JW, Stewart PS, Greenberg EP, Science, 284(5418), 1318 (1999)

[21] Hall-Stoodley L, Costerton J, Stoodley P, Nature Reviews Microbiology., 2, 95 (2004)

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[23] Choi O, Yu CP, Esteban Ferna´ ndez G, Hu Z, Water Research., 44, 6095 (2010)

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[27] Center for Biofilm Engineering, Standardized Biofilm Methods Team, CDC Biofilm Reactor Operator’s Manual (2003)

[28] Tilton R, Rosenberg B, Applied and Environmental Microbiology., 35, 1116 (1978)

[29] Kim J, Pitts B, Stewart P, Camper A, Yoon J, Antimicrobial Agents and Chemotherapy., 52, 1446 (2008)

[30] Choi O, Hu Z, Environmental Science and Technology., 42, 4583 (2008)

[31] Bae E, Park HJ, Lee J, Kim Y, Yoon J, Park K, Choi K, Yi J, Environmental Toxicology and Chemistry., 29, 2154 (2010)

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[33] Lee V, Li H, Lau T, Guevremont R, Siu K, Journal of the American Society for Mass Spectrometry., 9, 760 (1998)

[34] Hong SH, Jeong J, Shim S, Kang H, Kwon S, Ahn KH, Yoon J, Biotechnol. Bioeng., 100(2), 379 (2008)

[35] El Badawy AM, Luxton TP, Silva RG, Scheckel KG, Suidan MT, Tolaymat TM, Environmental Science and Technology., 44, 1260 (2010)