The effects of different loading procedures of magnetic nanoparticles (MNs) on the structure and physicochemical properties of cisplatin magnetic liposomes were investigated by X-ray diffraction, infrared spectroscopy, transmission electron microscopy, and fluorescence spectroscopy. 1, 2-Dipalmitoyl-sn-glycero-3-Phosphocholine based cisplatin magnetic liposomes were prepared using two different procedures. In procedure I, MNs were combined with phospholipids during film formation; MNs were embedded in a phospholipid bilayer. In procedure II, MNs were mixed with drugs during hydration and MNs were contained in an interior aqueous compartment. The encapsulation efficiency of cisplain and the content of MN in procedure I liposomes were 33.5% +/- 3.3% and 2.34 +/- 0.09 mg mL (1), respectively. It indicated that the deliberate MN loading into the liposome structure was not only successful using procedure I, but also superior over procedure II both in cisplatin encapsulation efficiency and MN content, which can promote the magnetic targeting effect of magnetic liposomes during delivering cisplatin.