화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.6, 1335-1338, June, 2013
DOI10.1007/s11814-013-0058-z  Export Citation
Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production
Jong Ho Lee, Sung Bong Kim, Hah Young Yoo, Ja Hyun Lee, Sung Ok Han1, Chulhwan Park2, and Seung Wook Kim†
  • Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea
  • 1College of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea
  • 2Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, Seoul 139-701, Korea
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Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
Keywords:Biodiesel;Enzymatic Process;Co-immobilization;Lipase
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