Generation of uniform agarose microwells for cell patterning by micromolding in capillaries

Heon-Ho Jeong; Ji-Hye Lee; Young-Moo Noh; Yun-Gon Kim; Chang-Soo Lee

Macromolecular Research, Vol.21, No.5, 534-540, May, 2013

DOI10.1007/s13233-013-1048-z Export Citation

Generation of uniform agarose microwells for cell patterning by micromolding in capillaries

Heon-Ho Jeong, Ji-Hye Lee, Young-Moo Noh, Yun-Gon Kim¹, and Chang-Soo Lee^†

Department of Chemical Engineering, Chungnam National University, Daejeon, 305-764, Korea
¹Department of Chemical Engineering, College of Engineering, Soongsil University, Seoul, 156-743, Korea

E-mail:

We described a simple and facile method to generate uniform agarose microwells on a polyelectrolyte (PEL) multilayer functionalized surface for efficient and reliable cell patterning. The PEL multilayers, composed of polyallylamine hydrochloride (PAH) and polystyrene sulfonate ammonium (PSS), provide an adhesive environment, which promotes cell proliferation in live cell-based assays. Agarose microwells, which are able to selectively isolate cells into individual compartments, are fabricated by micromolding the agarose solution in capillaries (MIMIC). Moreover, the fabricated agarose microwells are able to effectively form different shapes (e.g., circles, triangles, squares, and stars) and can isolate multiple cell types (e.g., HEK 293, NIH3T3, and HepG2). We also demonstrated how this technique can be used for the real-time monitoring of communication between primary neuronal cells in the agarose microwells.

Keywords:polyelectrolyte (PEL) multilayers;agarose microwells;micromolding in capillaries (MIMIC);neuronal cells;cell-cell interaction

[References]

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Willner I, Katz E, Angew. Chem.-Int. Edit., 39, 1180 (2000)
Shim HW, Lee JH, Choi HS, Lee CS, Korean Chem. Eng. Res., 46(6), 1100 (2008)
Song HM, Lee CS, Korean J. Chem. Eng., 25(6), 1467 (2008)
Falconnet D, Csucs G, Grandin HM, Textor M, Biomaterials, 27, 3044 (2006)
Khetani SR, Bhatia SN, Nat. Biotechnol., 26, 120 (2008)
Jeong HH, Lee SH, Kim JM, Kim HE, Kim YG, Yoo JY, Chang WS, Lee CS, Biosens. Bioelectron., 26, 351 (2010)
Wang C, Yap FL, Zhang Y, Colloids Surf. B: Biointerfaces, 46, 255 (2005)
Kim SK, Kim BG, Lee JH, Lee CS, Macromol. Res., 17(4), 232 (2009)
Lee JH, Kim HE, Im JH, Bae YM, Choi JS, Huh KM, Lee CS, Colloids Surf. B: Biointerfaces, 64, 126 (2008)
Shim HW, Lee JH, Hwang TS, Rhee YW, Bae YM, Choi JS, Han J, Lee CS, Biosens. Bioelectron., 22, 3188 (2007)
Whitesides GM, Ostuni E, Takayama S, Jiang XY, Ingber DE, Ann. Rev. Biomed. Eng., 3, 335 (2001)
Suh KY, Seong J, Khademhosseini A, Laibinis PE, Langer R, Biomaterials, 25, 557 (2004)
Choi CH, Lee JH, Hwang TS, Lee CS, Kim YG, Yang YH, Huh KM, Macromol. Res., 18(3), 254 (2010)
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Jeon NL, Choi IS, Xu B, Whitesides GM, Adv. Mater., 11(11), 946 (1999)
Lee S, Voros J, Langmuir, 21(25), 11957 (2005)
You Y, Yang H, Chung JW, Kim JH, Jung Y, Park SY, Angew. Chem.-Int. Edit., 49, 3757 (2010)
Yang L, Shirahata N, Saini G, Zhang F, Pei L, Asplund MC, Kurth DG, Ariga K, Sautter K, Nakanishi T, Smentkowski V, Linford MR, Langmuir, 25(10), 5674 (2009)
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[25] Zabow G, Assi F, Jenks R, Prentiss M, Appl. Phys. Lett., 80, 1483 (2002)

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