The main goal of the Bioprocess Technology working group is to overcome existing knowledge deficits in the bioprocess characterization of single-use bioreactors. The required methods have been investigated extensively, evaluated for their suitability for certain single-use bioreactors, and summarized into a basic structure in the so-called conceptual matrix. Development of standard operating procedures for the methods selected as well as their verification in interlaboratory tests guarantee that in the future a spectrum of unified and validated methods can be used to compare different bioreactor types objectively.