당지질계 미생물 계면활성제에 관한 연구(제1보); <i>Pseudomonas</i> sp. 13에 의한 Rhamnolipid의 생성에 있어 배지의 최적효과

이선주; 남기대

Journal of the Korean Industrial and Engineering Chemistry, Vol.4, No.1, 41-45, March, 1993

당지질계 미생물 계면활성제에 관한 연구(제1보); Pseudomonas sp. 13에 의한 Rhamnolipid의 생성에 있어 배지의 최적효과

Studies on the Glycolipid Biosurfactant(1) The Optimum Effect of Medium of Rhamnolipid by Pseudomonas sp.13

Rescently we are interested in the biosurfactant. Biosurfactant have a low toxcity and easily biodegradable compound. Pseudomonas sp. 13 was isolated from soil. This microorganism produced biosurfactant that consists of glycolipid R-1 and R-2. A time course study of fermentation indicated that the appearance of glycolipid in the fermentation broth the commencement of the stationary phase with the respect to biomass. The effect of variation of the media components such as amount of glucose, nitrogen, phosphate and metal ions has been investigated. The following values found to be optimum for biosurfactant production (glucose, 20 g/ℓ; carbon to nitrogen ratio, 40; carbon to phosphate, 18; FeSO₄·7H₂O 20㎎/ℓ).

[References]

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[1] Gooper DG, Zajic JE, Adv. Appl. Microbiol., 26, 229 (1980)

[2] Finnert WR, Singer ME, J. Biotechnol., 1, 47 (1983)

[3] Suzuki T, Tanaka K, Matsuhara I, Kinoshita S, Agric. Biol. Chem., 33, 1619 (1969)

[4] Kretschmer A, Bock H, Wagner F, Appl. Environ. Microbiol., 44, 170 (1982)

[5] Cooper DG, Paddock DA, Appl. Environ. Microbiol., 47, 173 (1984)

[6] Goldman S, Shabtai Y, Rubinovitz C, Rosenberg E, Gutnick DL, Appl. Environ. Microbiol., 44, 167 (1982)

[7] Hisatsaka K, Nakahama T, Sano N, Yamada K, Agric. Biol. Chem., 35, 686 (1971)

[8] Luis GA, Othmar K, Armin F, Appl. Environ. Microbiol., Aug., 301 (1984)

[9] Cooper DG, Microbiol. Sci., 3, 5 (1986)

[10] Jarvis EG, Johnson MJ, J. Am. Chem. Soc., 71, 4121 (1949)

[11] Hause G, Karnovsky ML, J. Bacteriol., 68, 645 (1961)

[12] Edwards JR, Hayashi JA, Arch. Biochem. Biophys., 111, 415 (1965)

[13] Itoch S, Suzuki T, Agric. Biol. Chem., 36, 2233 (1972)

[14] Itoch S, Homda H, J. Antibiotics, 24, 855 (1971)

[15] Dubois M, Gilles KA, Hamilton JK, Anal. Chem., 28, 350 (1956)

[16] Miller GL, Anal. Chem., 31, 142 (1959)

[17] Ramana KV, Karanth NG, J. Chem. Technol. Biotechnol., 45, 249 (1989)