Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

Irisappan Ganesh; Sambandam Ravikumar; Si Jae Park; Seung Hwan Lee; Soon Ho Hong

Korean Journal of Chemical Engineering, Vol.30, No.7, 1443-1447, July, 2013

DOI10.1007/s11814-013-0061-4 Export Citation

Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli

Irisappan Ganesh, Sambandam Ravikumar¹, Si Jae Park², Seung Hwan Lee³, and Soon Ho Hong^†

Department of Chemical Engineering, University of Ulsan, 93, Daehak-ro, Nam-gu, Ulsan 680-749, Korea
¹Department of Pharmaceutical Science and Technology, Catholic University of Daegu, 330, Geumrak 1-ri, Hayang-eup, Gyeongsan-si, Gyeongbuk 712-702, Korea
²Department of Environmental Engineering and Energy, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggido 449-728, Korea
³Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yuseong-gu, Daejeon 305-600, Korea

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The malate-pyruvate conversion pathway is catalyzed by two malic enzyme isomers, MaeA and MaeB. qRT-PCR was carried out under malate and pyruvate supplemented conditions to understand the dynamics of maeA and maeB gene expression. maeA expression was elevated by malate, and maeB expression was inhibited by levels of both malate and pyruvate higher than 0.1 mM. Green fluorescent protein (GFP) reporter plasmids were also constructed by integration of the maeA/maeB promoter with the gfp gene. We showed that maeA driven GFP expression was positively and negatively correlated with extracellular malate and pyruvate induction. In contrast, no significant changes in maeB driven GFP expression were observed under both malate and pyruvate supplemented conditions.

Keywords:Malate;Pyruvate;maeA;maeB;Green Fluorescent Protein;Escherichia coli

[References]

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Battat E, Peleg Y, Bercovitz A, Rokem JS, Goldberg I, Biotechnol.Bioeng., 37, 1108 (1991)
Pines O, Evenram S, Elnathan N, Battat E, Aharonov O, Gibson D, Goldberg I, Appl. Microbiol. Biotechnol., 46(4), 393 (1996)
Kawagoe M, Hyakumura K, Suye SI, Miki K, Naoe K, J. Ferment. Bioeng., 84(4), 333 (1997)
Taing O, Taing K, Eur. Food Res. Technol., 224, 343 (2007)
Rosenberg M, Mikova H, KristofIkova L, Lett. Appl. Microbiol., 29, 221 (1999)
Bressler E, Pines O, Goldberg I, Braun S, Biotechnol. Prog., 18(3), 445 (2002)
Li G, Yao D, Zong M, Eur. Polym. J., 44, 1123 (2008)
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Kwon YD, Lee SY, Kim P, J. Microbiol. Biotechnol., 16, 1448 (2006)
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Chang GG, Tong L, Biochem., 42, 12721 (2003)
Murai T, Tokushige M, Nagai J,Katsuki H, Biochem. Biophys. Res. Commun., 43, 875 (1971)
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Moon SY, Hong SH, Kim TY, Lee SY, Biochem. Eng. J., 40, 312 (2008)
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Thauer RK, Jungermann K, Decker K, Bact. Rev., 41, 100 (1977)
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[Referenced By]

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[2] Battat E, Peleg Y, Bercovitz A, Rokem JS, Goldberg I, Biotechnol.Bioeng., 37, 1108 (1991)

[3] Pines O, Evenram S, Elnathan N, Battat E, Aharonov O, Gibson D, Goldberg I, Appl. Microbiol. Biotechnol., 46(4), 393 (1996)

[4] Kawagoe M, Hyakumura K, Suye SI, Miki K, Naoe K, J. Ferment. Bioeng., 84(4), 333 (1997)

[5] Taing O, Taing K, Eur. Food Res. Technol., 224, 343 (2007)

[6] Rosenberg M, Mikova H, KristofIkova L, Lett. Appl. Microbiol., 29, 221 (1999)

[7] Bressler E, Pines O, Goldberg I, Braun S, Biotechnol. Prog., 18(3), 445 (2002)

[8] Li G, Yao D, Zong M, Eur. Polym. J., 44, 1123 (2008)

[9] Werpy T, Petersen G, Top value added chemicals from biomass, U.S. Department of Energy, Washington, DC (2004)

[10] Goldberg I, Rokem JS, Pines O, J. Chem. Technol. Biotechnol., 81(10), 1601 (2006)

[11] Engel CAR, Straathof AJJ, Zijlmans TW, van Gulik WM, van der Wielen LAM, Appl. Microbiol. Biotechnol., 78(3), 379 (2008)

[12] Millard CS, Chao YP, Liao JC, Donnelly MI, Appl. Environ.Microbiol., 62, 1808 (1996)

[13] Kwon YD, Lee SY, Kim P, J. Microbiol. Biotechnol., 16, 1448 (2006)

[14] Kim P, Laivenieks M, Vieille C, Zeikus JG, Appl. Environ.Microbiol., 70, 1238 (2004)

[15] Gokarn RR, Eiteman MA, Altman E, Appl. Environ. Microbiol., 66, 1844 (2000)

[16] Stols L, Donnelly MI, Appl. Environ. Microbiol., 63, 2695 (1997)

[17] Hong SH, Lee SY, Biotechnol. Bioeng., 74(2), 89 (2001)

[18] Chang GG, Tong L, Biochem., 42, 12721 (2003)

[19] Murai T, Tokushige M, Nagai J,Katsuki H, Biochem. Biophys. Res. Commun., 43, 875 (1971)

[20] Bologna FP, Andreo CS, Drincovich MF, J. Bacteriol., 189, 5937 (2007)

[21] Wang B, Wang P, Zheng E, Chen X, Zhao H, Song P, Su R, Li X, Zhu G, J. Microbiol., 49, 797 (2011)

[22] Miller WG, Leveau JHJ, Lindow SE, Mol. Plant Microbe In., 13, 1243 (2000)

[23] Sambrook J, Russell DW, Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1 (2001)

[24] Eleaume H, Jabbouri S, J. Microbiol. Methods., 59, 363 (2004)

[25] Moon SY, Hong SH, Kim TY, Lee SY, Biochem. Eng. J., 40, 312 (2008)

[26] Zelle RM, de Hulster E, van Winden WA, de Waard P, Dijkema C, Winkler AA, Geertman JMA, van Dijken JP, Pronk JT, van Maris AJA, Appl. Environ. Microbiol., 74, 2766 (2008)

[27] Zhang X, Wang X, Shanmugam KT, Ingram LO, Appl. Environ.Microbiol., 77, 427 (2011)

[28] Thauer RK, Jungermann K, Decker K, Bact. Rev., 41, 100 (1977)

[29] Goldberg RN, Tewari YB, Bell D, Fazio K, J. Phys. Chem. Ref. Data., 22, 515 (1993)

[30] McCleary WR, Stock JB, J. Biol. Chem., 269, 31567 (1994)