Growth kinetics of an indigenous mixed microbial consortium during methylene chloride degradation in a batch reactor

Rajamanickam Ravi; Ligy Philip; Tyagarajan Swaminathan

Korean Journal of Chemical Engineering, Vol.30, No.9, 1770-1774, September, 2013

DOI10.1007/s11814-013-0099-3 Export Citation

Growth kinetics of an indigenous mixed microbial consortium during methylene chloride degradation in a batch reactor

Rajamanickam Ravi^†, Ligy Philip¹, and Tyagarajan Swaminathan²

Department of Chemical Engineering, Annamalai University, Chidambaram, Tamil Nadu-608001, India
¹Department of Civil Engineering, Indian Institute of Technology, Madras, India
²Department of Chemical Engineering, Indian Institute of Technology, Madras, India

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Biodegradation of methylene chloride by a mixed microbial culture, isolated from a common sewage treatment plant, was investigated in a batch system. Batch experiments were performed at room temperature (27 ℃) and pH value of 7. The methylene chloride concentration in growth media varied from 25 mgl^(-1) to 250mgl^(-1). A maximum observed degradation was 1 mgl.1h.1 at 100 mgl.1 of methylene chloride. The culture followed substrate inhibition kinetics and specific growth rate were fitted to different substrate inhibition models (Haldane, Aiba and Edwards models) by MATLAB 7.1@. Among all models, Haldane was found to better fit with root mean square of 0.947. The biokinetic constants estimated using these models show good potential of the mixed microbial culture in methylene Chloride degradation. Escherichia coli and Staphylococcus aureus are predominant microbes present in the mixed culture.

Keywords:Mixed Culture;Dichloromethane;Biodegradation;Kinetics;Inhibition Models

[References]

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[1] Brunner W, Staub D, Leisinger T, Appl. Environ. Microb., 40, 950 (1980)

[2] Freedman DL, Gossett JM, Appl. Environ. Microb., 57, 2847 (1991)

[3] Herbst B, Wiseman U, Water Res., 30(5), 1069 (1996)

[4] Wang JD, Chen JM, Chem. Eng. J., 123(3), 103 (2006)

[5] Trotsenko YA, Doronina NV, Tourova TP, Kuznetzov BB, Leisinger T, Systematic and Appl. Microbiol., 23, 210 (2000)

[6] Goodwin KD, Schaefer JK, Oremland RS, Appl. Environ.Microb., 164, 4629 (1998)

[7] Doris KS, Thomas L, J. Bacteriol., 162(2), 676 (1985)

[8] Van Pee KH, Unversucht S, Chemosphere., 52, 299 (2003)

[9] Hartmans DS, Tramper J, Bioprocess Eng., 6, 83 (1991)

[10] Guo GL, Tseng DH, Huang SL, Biotechnol. Lett., 23(20), 1653 (2001)

[11] Krausova VI, Robb FT, Gonzalez JM, J. Microbiol. Methods., 54, 419 (2003)

[12] Nagamani A, Soligala R, Lowry M, African J. Biotechnol., 8(20), 5449 (2009)

[13] MacFaddin JF, Biochemical tests for identification of medical bacteria, Williams and Wilkins, London (2000)

[14] Gokulakrishanan S, Sathyanarayana Gummadi N, Process Biochem., 41(6), 1417 (2006)

[15] Wu SJ, Zhang LL, Wang JD, Chen JM, Appl. Microbiol. Biotechnol., 76(6), 1289 (2007)

[16] Qinaruguri Y, Hiroshi N, Wataru H, Yukie O, Resources Processing., 58, 47 (2011)

[17] Shijin WU, Xiang YU, Zhihang HU, Zhang L, Chen J, J.Environ. Sci., 21, 1276 (2009)

[18] Dev S, Mukherjee S, J. Water Reuse and Desalination., 2, 149 (2012)

[19] Dev S, Mukherjee S, Int. J. Water Res. Environ.Eng., 2(3), 40 (2010)

[20] Reardon KF, Mosteller DC, Rogers JDB, Biotechnol.Bioeng., 69(4), 387 (2000)

[21] Shim H, Yang ST, J. Biotechnol., 67, 99 (1999)