Properties of Jatropha seed oil from seven different countries were evaluated. Jatropha curcas oil was extracted using n-hexane as the solvent in the Soxhlet extraction method. The oil obtained from hexane extraction was different from each country and varied from 40.03 to 48.37% (w/w). The highest oil yield (48.37%) was obtained from the seed of Vietnam origin. The oil from Indonesian origin obtained higher free fatty acid content (1.906%), whereas South African seeds obtained the lowest free fatty acid (0.43%). Seed oil from Indonesian origin had higher iodine value (216.20 mg/g oil), whereas it was lower (138.12 mg/g oil) in South African seed oil. The saponification value of seed oil was the highest (197.14 mg KOH/g) in Indian seeds and the lowest (189.46 mg KOH/g) in Philippine origin. The pattern of the physiochemical properties of different seed sources under this study showed a difference in oil properties irrespective of seed origin.