An experimental and numerical study has been carried out in order to investigate mixed and natural convection heat transfer in a two-dimensional enclosure. A discrete isothermal heat source is located at one of the vertical walls. Also, two ventilation ports are at the bottom and on top of the opposite wall. A forced flow condition was imposed by providing an inlet of air at the bottom port. A Mach-Zehnder interferometer was used to visualize the temperature field within the enclosure and to determine the local and average heat transfer characteristics of the heat source. Five heater positions on the vertical wall and different Rayleigh numbers (4.5 x 10(5) to 1.15 x 10(6)) and Reynolds numbers (120 to 1600) were considered in the experiments. A finite volume code has been developed based on the SIMPLE algorithm and hybrid discretization scheme for the numerical study. It is observed that the interaction of natural convection with the forced flow leads to various flow fields depending on the Richardson number, Reynolds number and the heater position. Also, results show different trends for variation of the average Nusselt number with the heater position at low and high Reynolds numbers. An optimum position for the heat source, at which the maximum heat transfer is achieved, exists for high Reynolds numbers and has been found to be at the middle of the vertical wall.