The distribution coefficient was measured at 25 degrees C for beta-carotene in binary aqueous immiscible systems of organic solvents : toluene, cyclohexane, hexane, methylcyclohexane, and CHCl3. The values of the distribution coefficients of the solvents examined rise in the following order : hexane < cyclohexane < methylcyclohexane < toluene < chloroform. Chloroform was found unsuitable as a beta-carotene solvent, as it forms a complex or most likely reacts with the latter.