The widespread use of polymers from renewable sources is currently unachievable, not only by economic constraints but by the limiting properties of this type of material. Several strategies have been developed to overcome this problem; one of the most extensively explored is the use of nanofillers to improve the biopolymer's properties such as stiffness, impact strength and gas barrier, amongst others. However, several gaps in understanding the morphology formation of these systems still exist. This study evaluates the use of glycerol as an auxiliary additive during production of polylactide/montmorillonite nanocomposites. In this study, the presence of glycerol seems to influence the nanocomposites' morphology, influencing the clay exfoliation in some cases, and the mechanical properties. On the other hand, the presence of glycerol seems to induce a greater molecular weight reduction during twin screw extrusion.