The localization of nanoclay particles dispersed in the oil phase of a model oil-in-water emulsion depends on the wetting property of layered nanoparticles. Investigation at a single droplet interface shows that nanoclay is located at different interfacial regions depending on the hydrophilic property of the nanoclay surface. Hydrophobic nanoclays do not present Pickering phenomena at the interface and hardly form an interfacial layer. Hydrophilic nanoclay particles quickly move to the interface and form a Pickering interface with a high interfacial shear modulus. With surfactant, poor hydrophilic nanoclays can be located at the interface due to improvement of the wetting behavior caused by the surfactants dissolved in the aqueous continuous phase. With ionic molecules changing the wetting behavior of particles, the interfacial localization of nanoclays can be controlled and improve the mechanical property of emulsion.