파클리탁셀 정제 효율에 미치는 실리카-알루미나 흡착제의 영향

오현정; 정경열; 김진현; Hyeon-Jeong Oh,; Kyeong Youl Jung; Jin-Hyun Kim

Clean Technology, Vol.19, No.3, 342-346, September, 2013

DOI10.7464/ksct.2013.19.3.342 Export Citation

파클리탁셀 정제 효율에 미치는 실리카-알루미나 흡착제의 영향

Effect of Silica-Alumina Adsorbent on the Efficiency of Paclitaxel Purification

오현정, 정경열 ^†, 김진현 ^†

공주대학교 화학공학부, 331-717 충남 천안시 서북구 천안대로 1223-24

Hyeon-Jeong Oh,, Kyeong Youl Jung^†, and Jin-Hyun Kim^†

Department of Chemical Engineering, Kongju National University, 1223-24 Cheonan-daero, Seobuk-gu, Cheonan-si, Chungnam 331-717, Korea

E-mail:,

초록

본 연구에서는 분무열분해(spray pyrolysis)에 의해 다양한 혼합비의 다공성 메조기공 실리카-알루미나 흡착제를 제조하여 항암물질 파클리탁셀(paclitaxel)의 정제에 이용하였다. 흡착제 처리와 헥산 침전을 수행한 결과, S0.5A0.5 (Si/Al 몰비 = 0.5/0.5)을 사용하였을 때 가장 높은 순도 (~27%)와 수율 (~63%)의 파클리탁셀을 얻을 수 있었다. 이러한 불순물(타르 및 왁스 성분 포함) 제거 효과는 흡착제에 붙은 유기물의 TGA 정량 분석 결과로도 확인할 수 있었다.

Several types of mesoporous silica-alumina adsorbents with varied SiO2/Al2O3 ratios were prepared by spray pyrolysis and were used for the purification of the anticancer agent paclitaxel. When S0.5A0.5 (Silica/Alumina molar ratio = 0.5/0.5) was used, the highest purity (~27%) and yield (~63%) of paclitaxel were obtained from adsorbent treatment and hexane precipitation. Removal of impurities was confirmed by TGA analysis of the organic substances that were adsorbed to the adsorbent.

Keywords:Paclitaxel;Silica-alumina;Adsorbent;Purification

[References]

Kim JH, Kor. J. Biotechnol. Bioeng., 21, 1 (2006)
Georgiev MI, Weber J, Maciuk A, Appl. Microbiol. Biotechnol., 83(5), 809 (2009)
Kolewe ME, Gaurav V, Roberts SC, Mol. Pharm., 5, 243 (2008)
Han MG, Kim JH, Biotechnol. Bioproc. Eng., 17, 1018 (2012)
Lee JY, Kim JH, Process Biochem., 47, 2388 (2012)
Sim HA, Lee JY, Kim JH, Sep.Purif. Technol., 89, 112 (2012)
Castor TP, “Method and apparatus for isolating therapeutic compositions from source materials,” U.S. Patent No. 5,750,709 (1998)
ElSohly HN, Croom EM, ElSohly MA, Mc-Chesney JD, “Methods and compositions for isolating taxanes," U.S. Patent No. 5,618,538 (1997)
Rao KV, “Method for the isolation and purification of taxol and its natural analogues,” U.S. Patent No. 5,670,673 (1997)
Pyo SH, Park HB, Song BK, Han BH, Kim JH, Process Biochem., 39, 1985 (2004)
Pyo SH, Song BK, Ju CH, Han BH, Choi HJ, Process Biochem., 40, 1113 (2005)
Kim JH, Kang IS, Choi HK, Hong SS, Lee HS, Process Biochem., 37, 679 (2002)
Oh HJ, Jang HR, Jung KY, Kim JH, Process Biochem., 47, 331 (2012)
Oh HJ, Jung KY, Kim JH, Korean J. Microbiol. Biotechnol., “Evaluation of Mesoporous Alumina Adsorbent for Purification of Paclitaxel,” In press (2013)
Gamborg OL, Miller RA, Ojima K, Exp. Cell Res., 50, 151 (1968)
Choi HK, Adams TL, Stahlhut RW, Kim SI, Yun JH, Song BK, Kim JH, Hong SS, Lee HS, “Method for mass production of taxol by semi-continuous culture with taxus chinensis cell culture,” U.S. Patent No.5,871,979 (1999)
Kim JH, KSBB J., 24, 212 (2009)
Gregg SJ, Sing KSW, Adsorption, Surface Area and Porosity, 2nd ed., Academic Press, NY, 41 (1982)
Lee JY, Kim JH, Sep. Purif. Technol., 103, 8 (2013)
Oh HJ, Jang HR, Jung KY, Kim JH, Microp. Mesop. Mater., 180, 109 (2013)

[Related Articles]

[1] Kim JH, Kor. J. Biotechnol. Bioeng., 21, 1 (2006)

[2] Georgiev MI, Weber J, Maciuk A, Appl. Microbiol. Biotechnol., 83(5), 809 (2009)

[3] Kolewe ME, Gaurav V, Roberts SC, Mol. Pharm., 5, 243 (2008)

[4] Han MG, Kim JH, Biotechnol. Bioproc. Eng., 17, 1018 (2012)

[5] Lee JY, Kim JH, Process Biochem., 47, 2388 (2012)

[6] Sim HA, Lee JY, Kim JH, Sep.Purif. Technol., 89, 112 (2012)

[7] Castor TP, “Method and apparatus for isolating therapeutic compositions from source materials,” U.S. Patent No. 5,750,709 (1998)

[8] ElSohly HN, Croom EM, ElSohly MA, Mc-Chesney JD, “Methods and compositions for isolating taxanes," U.S. Patent No. 5,618,538 (1997)

[9] Rao KV, “Method for the isolation and purification of taxol and its natural analogues,” U.S. Patent No. 5,670,673 (1997)

[10] Pyo SH, Park HB, Song BK, Han BH, Kim JH, Process Biochem., 39, 1985 (2004)

[11] Pyo SH, Song BK, Ju CH, Han BH, Choi HJ, Process Biochem., 40, 1113 (2005)

[12] Kim JH, Kang IS, Choi HK, Hong SS, Lee HS, Process Biochem., 37, 679 (2002)

[13] Oh HJ, Jang HR, Jung KY, Kim JH, Process Biochem., 47, 331 (2012)

[14] Oh HJ, Jung KY, Kim JH, Korean J. Microbiol. Biotechnol., “Evaluation of Mesoporous Alumina Adsorbent for Purification of Paclitaxel,” In press (2013)

[15] Gamborg OL, Miller RA, Ojima K, Exp. Cell Res., 50, 151 (1968)

[16] Choi HK, Adams TL, Stahlhut RW, Kim SI, Yun JH, Song BK, Kim JH, Hong SS, Lee HS, “Method for mass production of taxol by semi-continuous culture with taxus chinensis cell culture,” U.S. Patent No.5,871,979 (1999)

[17] Kim JH, KSBB J., 24, 212 (2009)

[18] Gregg SJ, Sing KSW, Adsorption, Surface Area and Porosity, 2nd ed., Academic Press, NY, 41 (1982)

[19] Lee JY, Kim JH, Sep. Purif. Technol., 103, 8 (2013)

[20] Oh HJ, Jang HR, Jung KY, Kim JH, Microp. Mesop. Mater., 180, 109 (2013)