Fabrication of poly(vinyl acetate)/polysaccharide biocomposite nanofibrous membranes for tissue engineering

Hyo-Geun Jeong; Young-Eun Kim; Young-Jin Kim

Macromolecular Research, Vol.21, No.11, 1233-1240, November, 2013

DOI10.1007/s13233-013-1155-x Export Citation

Fabrication of poly(vinyl acetate)/polysaccharide biocomposite nanofibrous membranes for tissue engineering

Hyo-Geun Jeong, Young-Eun Kim, and Young-Jin Kim^†

Department of Biomedical Engineering, Catholic University of Daegu, Gyeongbuk, 712-702, Korea

E-mail:

To develop a new potential scaffold, biocomposite nanofibrous membranes composed of poly(vinyl acetate) (PVAc)/polysaccharide were prepared using an electrospinning process. The resulting nanofibrous membranes exhibited a fully interconnected pore structure and their average diameter was influenced by a change of conductivity and viscosity of the electrospinning solutions due to the addition of polysaccharides. According to the X-ray diffraction (XRD) results, the crystalline phase of PVAc was hardly affected by the addition of polysaccharides. However, the mechanical properties were markedly changed by the addition of polysaccharides. In addition, the water contact angle and zeta potential were slightly affected by the use of polysaccharides. Cytocompatibility results showed that cell attachment improved by the addition of chitosan, while cell proliferation enormously increased by blending pullulan. Therefore, PVAc/polysaccharide biocomposite nanofibrous membranes appear to be promising biomaterials for tissue engineering.

Keywords:polysaccharide;biocomposite;nanofibrous membrane;tissue engineering

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[Referenced By]

[1] Deville S, Saiz E, Tomsia AP, Biomaterials, 27, 5480 (2006)

[2] Turco G, Marsich E, Bellomo F, Semeraro S, Donati I, Brun F, Grandolfo M, Accardo A, Paoletti S, Biomacromolecules, 10(6), 1575 (2009)

[3] Nehrer S, Breinan HA, Ramappa A, Young G, Shortkroff S, Louie LK, Sledge CB, Yannas IV, Spector M, Biomaterials, 18, 769 (1997)

[4] Chen ZG, Wang PW, Wei B, Mo XM, Cui FZ, Acta Biomater., 6, 372 (2010)

[5] Ha YM, Amna T, Kim MH, Kim HC, Hassan MS, Khil MS, Colloids Surf. B: Biointerfaces, 102, 795 (2013)

[6] Smith LA, Ma PX, Colloids Surf. B: Biointerfaces, 39, 125 (2004)

[7] Jose MV, Thomas V, Johnson KT, Dean DR, Nyairo E, Acta Biomater., 5, 305 (2009)

[8] Ciardelli G, Chiono V, Vozzi G, Pracella M, Ahluwalia A, Barbani N, Cristallini C, Giusti P, Biomacromolecules, 6(4), 1961 (2005)

[9] Nair LS, Laurencin CT, Prog. Polym. Sci, 32, 762 (2007)

[10] Koh HS, Yong T, Chan CK, Ramakrishna S, Biomaterials, 29, 3574 (2008)

[11] Cheng M, Deng J, Yang F, Gong Y, Zhao N, Zhang X, Biomaterials, 24, 2871 (2003)

[12] Sabir MI, Xu X, Li L, J. Mater. Sci. -Mater. Med., 44, 5713 (2009)

[13] Novoa GAG, Heinamaki J, Mirza S, Antikainen O, Colarte AI, Paz AS, Yliruusi J, Eur. J. Pharm. Biopharm., 59, 343 (2005)

[14] Sadato A, Taki W, Ikada Y, Nakahara I, Yamashita K, Matsumoto K, Tanaka M, Kikuchi H, Doi Y, Noguchi T, Neuroradiology, 36, 634 (1994)

[15] Muzzarelli RAA, Mar. Drugs, 8, 292 (2010)

[16] Kean T, Thanou M, Adv. Drug Deliv. Rev., 62, 3 (2010)

[17] Nguyen S, Winnik FM, Buschmann MD, Carbohydr. Polym., 75, 528 (2009)

[18] Dash M, Chiellini F, Ottenbrite RM, Chiellini E, Prog. Polym. Sci, 36, 981 (2011)

[19] Leathers TD, Appl. Microbiol. Biotechnol., 62(5-6), 468 (2003)

[20] Shingel KI, Carbohydr. Res., 339, 447 (2004)

[21] Autissier A, Visage CL, Pouzet C, Chaubet F, Letourneur D, Acta Biomater., 6, 3640 (2010)

[22] Fragonas E, Valente M, Pozzi-Mucelli M, Toffanin R, Rizzo R, Silvestri F, Vittur F, Biomaterials, 21, 795 (2000)

[23] Dar A, Shachar M, Leor J, Cohen S, Biotechnol. Bioeng., 80(3), 305 (2002)

[24] Ueyama Y, Ishikawa K, Mano T, Koyama T, Nagatsuka H, Suzuki K, Ryoke K, Biomaterials, 23, 2027 (2002)

[25] Hashimoto T, Suzuki Y, Tanihara M, Kakimaru Y, Suzuki K, Biomaterials, 25, 1407 (2004)

[26] Amarnath LP, Srinivas A, Ramamurthi A, Biomaterials, 27, 1416 (2006)

[27] Jia YT, Gong J, Gu XH, Kim HY, Dong J, Shen XY, Carbohydr. Polym., 67, 403 (2007)

[28] Kim YJ, Bae HI, Kwon OK, Choi MS, Int. J. Biol. Macromol., 45, 65 (2009)

[29] Wei S, Pintus V, Schreiner M, J. Anal. Appl. Pyrolysis, 97, 158 (2012)

[30] Han J, Zhou Z, Yin R, Yang D, Nie J, Int. J. Biol. Macromol., 46, 199 (2010)

[31] Sugumaran KR, Gowthami E, Swathi B, Elakkiya S, Srivastava SN, Ravikumar R, Gowdhaman D, Ponnusami V, Carbohydr. Polym., 92, 697 (2013)

[32] Chen JP, Su CH, Acta Biomater., 7, 234 (2011)

[33] Ahmad J, Hagg MB, J. Membr. Sci., 427, 73 (2013)

[34] Rubilar JF, Cruz RMS, Silva HD, Vicente AA, Khmelinskii I, Vieira MC, J. Food Eng., 115(4), 466 (2013)

[35] Wei JH, Yoshinari M, Takemoto S, Hattori M, Kawada E, Liu BL, Oda Y, J. Biomed. Mater. Res. Part B: Appl. Biomater., 81B, 66 (2007)

[36] Yeo M, Lee H, Kim G, Biomacromolecules, 12(2), 502 (2011)

[37] Wu F, Liu C, O’Neill B, Wei J, Ngothai Y, Appl. Surf. Sci., 258, 7859 (2012)

[38] Chen H, Yu J, Liu S, Gu P, Int. J. Biol. Macromol., 48, 13 (2011)

[39] Zhu YB, Gao CY, Liu XY, Shen JC, Biomacromolecules, 3(6), 1312 (2002)

[40] Zimmermann H, Zimmermann D, Reuss R, Feilen PJ, Manz B, Katsen A, Weber M, Ihmig FR, Ehrhart F, Gebner P, Behringer M, Steinbach A, Wegner LH, Sukhorukov VL, Vasquez JA, Schneider S, Weber MM, Volke F, Wolf R, Zimmermann U, J. Mater. Sci. -Mater. Med., 16, 491 (2005)

[41] Marguerite R, Polym. Int., 57, 397 (2008)