Sprayable powder of hyaluronate embedded in mildly cross-linked alginate as a post-surgical tissue adhesion barrier

Se Heang Oh; Seung Yeon Na; Kyu Sang Song; Jin Ho Lee

Macromolecular Research, Vol.21, No.11, 1263-1269, November, 2013

DOI10.1007/s13233-013-1163-x Export Citation

Sprayable powder of hyaluronate embedded in mildly cross-linked alginate as a post-surgical tissue adhesion barrier

Se Heang Oh, Seung Yeon Na, Kyu Sang Song¹, and Jin Ho Lee^†

Department of Nanobiomedical Science & WCU Research Center, Dankook University, Chungnam, 330-714, Korea
¹Department of Pathology, School of Medicine, Chungnam National University, Daejeon, 301-131, Korea

E-mail:

Hyaluronate (HA) powder was embedded in mildly cross-linked alginate (mcALG) for the preperation of the sprayable tissue adhesion barrier. The HA/mcALG powder could easily cover a peritoneal tissue defect site without spatial restriction (by air spraying) and effectively conceal the injured tissues without migration (by muco-adhesiveness of HA). It also remained at the applied area during wound healing (by HA embedding in the network structure of cross-linked ALG). The tissue adhesion prevention ability of HA/mcALG was evaluated and compared with each HA or mcALG powder through an animal study using a rat model. The HA/mcALG powder was highly effective in preventing peritoneal tissue adhesion and did not show abnormal tissue responses during wound healing. Based on these observations, the HA/mcALG powder is expected to be a good candidate for sprayable tissue adhesion barriers in clinical applications.

Keywords:tissue adhesion;tissue adhesion barrier;hyaluronate;alginate;sprayable powder

[References]

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diZerega GS, Fertil. Steril., 61, 219 (1994)
Al-Musawi D, Thompson JN, Gynaecol. Endosc., 10, 123 (2001)
Liu Y, Shu XZ, Prestwich GD, Fertil. Steril., 87, 940 (2007)
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Urman B, Gomel V, Fertil. Steril., 56, 568 (1991)
Cho WJ, Oh SH, Kim IG, Lee CS, Lee JH, Tissue Eng. Regen. Med., 7, 49 (2010)
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Johns DB, Rodgers KE, Donahue WD, Kiorpes TC, diZerega GS, Fertil. Steril., 68, 37 (1997)
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Li Y, Li H, Shu XZ, Gray SD, Prestwich GD, Fertil. Steril., 83, 1275 (2005)
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Oh SH, Kim JK, Song KS, Noh SM, Ghil SH, Yuk SH, Lee JH, J. Biomed. Mater. Res. A, 72A, 306 (2005)
Shirai Y, Hashimoto K, Irie S, Appl. Microbiol. Biotechnol., 31, 342 (1989)
Jaacobi Y, Israel AA, Goldberg EP, J. Surg. Res., 55, 422 (1993)
Chamberlain AHL, Angell P, Campbell HS, Br. Corrosion J., 23, 197 (1988)
Lee JH, Kim WG, Kim SS, Lee JH, Lee HB, J. Biomed. Mater. Res., 36, 200 (1997)
Ellis H, Harrison W, Hugh TB, Br. J. Surg., 52, 471 (1965)
Hubbard TB, Khan MZ, Carag VR, Albites VE, Hricko GM, Ann. Surg., 165, 908 (1967)
Bulpitt P, Aeschlimann D, J. Biomed. Mater. Res., 47, 152 (1999)
Bucke C, Methods Enzymol., 135, 175 (1987)
Cho SH, Oh SH, Lee JH, J. Biomater. Sci.-Polym. Ed., 16, 933 (2005)
Kim GH, Kang YM, Kang KN, Kim DY, Kim HJ, Tissue Eng. Regen. Med., 8, 1 (2011)
Moyer KE, Davis A, Saggers GC, Mackay DR, Ehrlich HP, Exp. Mol. Pathol., 72, 10 (2002)

[Referenced By]

[1] De Laco PA, Stefanetti M, Pressato D, Piana S, Dona M, Pavesio A, Bovicelli L, Fertil. Steril., 69, 318 (1998)

[2] Yeo Y, Kohane DS, Eur. J. Pharm. Biopharm., 68, 57 (2008)

[3] Bruggmann D, Tchartchian G, Wallwiener M, Munstedt K, Tinneberg HR, Hackethal A, Dtsch. Arztebl. Int., 107, 769 (2010)

[4] Menzies D, Ellis H, Ann. R. Coll. Surg. Engl., 72, 60 (1990)

[5] Ray NF, Denton WG, Thamer M, Henderson SC, Perry S, J. Am. Coll. Surg., 186, 1 (1998)

[6] Falk K, Lindman B, Bengmark S, Larsson K, Holmdahl L, Biomaterials, 22, 2185 (2001)

[7] Brochhausen C, Schmitt VH, Rajab TK, Planck CNE, Kramer B, Wallwiener M, Hierlemann H, Kirkpatrick CJ, J. Biomed. Mater. Res. A, 98A, 143 (2011)

[8] Diamond MP, DeCherney AH, Microsurgery, 8, 103 (1987)

[9] diZerega GS, Fertil. Steril., 61, 219 (1994)

[10] Al-Musawi D, Thompson JN, Gynaecol. Endosc., 10, 123 (2001)

[11] Liu Y, Shu XZ, Prestwich GD, Fertil. Steril., 87, 940 (2007)

[12] Lauder CIW, Garcea G, Strickland A, Maddern GJ, Dig. Surg., 27, 347 (2010)

[13] Habara T, Nakatsuka M, Konishi H, Asagiri K, Noguchi S, Kudo T, J. Biomed. Mater. Res., 61, 628 (2002)

[14] Adhesion Study Group, Fertil. Steril., 40, 612 (1983)

[15] Urman B, Gomel V, Fertil. Steril., 56, 568 (1991)

[16] Cho WJ, Oh SH, Kim IG, Lee CS, Lee JH, Tissue Eng. Regen. Med., 7, 49 (2010)

[17] Park JS, Cha SJ, Kim BG, Choi YS, Kwon GY, Kang H, An SS, J. Surg. Res., 168, 49 (2011)

[18] Gerecht S, Burdick JA, Ferreira LS, Townsend SA, Langer R, Vunjak-Novakovic G, Proc. Natl. Acad. Sci. U.S.A., 104, 11298 (2007)

[19] Shepard S, Becker H, Hartmann JX, Ann. Plast. Surg., 36, 65 (1996)

[20] King SR, Hickerson WL, Proctor KG, Surgery, 109, 76 (1991)

[21] Laurent TC, Fraser JR, Faseb J., 6, 2397 (1992)

[22] Peck LS, Goldberg EP, in Peritoneal Surgery, diZerega GS, Ed., Springer, New York, 2000, pp 499-520.

[23] Amiel D, Ishizue K, Billings E, Wiig M, Berg JV, Akeson WH, Gelberman R, J. Hand Surg. Am., 14, 837 (1989)

[24] Hagberg L, Gerdin B, J. Hand Surg. Am., 17, 935 (1992)

[25] Burns JW, Skinner K, Colt J, Sheidlin A, Bronson R, Yaacobi Y, Goldberg EP, J. Surg. Res., 59, 644 (1995)

[26] Chen WY, Abatangelo G, Wound Repair Regen., 7, 79 (1999)

[27] Johns DB, Rodgers KE, Donahue WD, Kiorpes TC, diZerega GS, Fertil. Steril., 68, 37 (1997)

[28] Yeo Y, Highley CB, Bellas E, Ito T, Marini R, Langer R, Kohane DS, Biomaterials, 27, 4698 (2006)

[29] Li Y, Li H, Shu XZ, Gray SD, Prestwich GD, Fertil. Steril., 83, 1275 (2005)

[30] De Iaco PA, Stefanetti M, Pressato D, Piana S, Dona M, Pavesio A, Bovicelli L, Fertil. Steril., 69, 318 (1998)

[31] Oh SH, Kim JK, Song KS, Noh SM, Ghil SH, Yuk SH, Lee JH, J. Biomed. Mater. Res. A, 72A, 306 (2005)

[32] Shirai Y, Hashimoto K, Irie S, Appl. Microbiol. Biotechnol., 31, 342 (1989)

[33] Jaacobi Y, Israel AA, Goldberg EP, J. Surg. Res., 55, 422 (1993)

[34] Chamberlain AHL, Angell P, Campbell HS, Br. Corrosion J., 23, 197 (1988)

[35] Lee JH, Kim WG, Kim SS, Lee JH, Lee HB, J. Biomed. Mater. Res., 36, 200 (1997)

[36] Ellis H, Harrison W, Hugh TB, Br. J. Surg., 52, 471 (1965)

[37] Hubbard TB, Khan MZ, Carag VR, Albites VE, Hricko GM, Ann. Surg., 165, 908 (1967)

[38] Bulpitt P, Aeschlimann D, J. Biomed. Mater. Res., 47, 152 (1999)

[39] Bucke C, Methods Enzymol., 135, 175 (1987)

[40] Cho SH, Oh SH, Lee JH, J. Biomater. Sci.-Polym. Ed., 16, 933 (2005)

[41] Kim GH, Kang YM, Kang KN, Kim DY, Kim HJ, Tissue Eng. Regen. Med., 8, 1 (2011)

[42] Moyer KE, Davis A, Saggers GC, Mackay DR, Ehrlich HP, Exp. Mol. Pathol., 72, 10 (2002)