화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.12, 2119-2126, December, 2013
DOI10.1007/s11814-013-0161-1  Export Citation
Advances in cultivation and processing techniques for microalgal biodiesel: A review
Mizhang Xiao1, 2, Hyun-Jae Shin1, †, and Qiuhua Dong2
  • 1Department of Chemical and Biochemical Engineering, Chosun University, Gwangju 501-759, Korea
  • 2School of Life Science & Technology, Huazhong University of Science & Technology, Wuhan 430074, P. R. China
E-mail:
The key technologies for producing microalgal biodiesel include microalgae screening, economical cultivation, and efficient methods in lipid extraction and conversion. Recent advances in microalgae cultivation, lipid extraction, and biodiesel preparation are reviewed in this work, with emphasis on photosynthetic metabolisms, separation efficiency and catalytic kinetics. The mutual exclusion between lipid accumulation and fast growth limits total lipid productivity, while only triglycerides in neutral lipids are converted to biodiesel through transesterification. The hurdles in large scale culture and low neural lipids yield are discussed, as well as the relationship between high unsaturation and fuel properties. This review aims to provide technical information to guide strain screening and lipid conversion for microalgal biodiesel industry.
Keywords:Microalgal Oil;Poly-unsaturated Fatty Acids (PUFAs);Biodiesel;Lipid Extraction;Purification
  1. Lai JQ, Hu ZL, Wang PW, Yang Z, Fuel, 95(1), 329 (2012)
  2. Amaro HM, Guedes AC, Malcata FX, Appl. Energy., 10, 3402 (2011)
  3. Lee YK, J. Appl. Phycology., 13, 307 (2001)
  4. Bozbas K, Renew. Sustain. Energy Rev., 12, 542 (2008)
  5. Canakci M, Sanli H, J. Ind. Microbiol. Biotechnol., 35, 431 (2008)
  6. Demirbas A, Prog. Energy Combust. Sci., 1, 1 (2007)
  7. Krawczyk T, Inform., 8, 801 (1996)
  8. Hossain A, Salleh A, Boyce AN, Naqiuddin M, Am. J. Biochem. Biotechnol., 3, 250 (2008)
  9. Hu Q, Sommerfeld M, Jarvis E, Seibert M, Darzins A, Plant J., 54, 621 (2008)
  10. Schenk PM, Hall SRT, Stephens E, Marx UC, Mussgnug JH, Posten C, Hankamer B, Bioenergy Res., 1, 20 (2008)
  11. Widjaja A, Chien CC, Ju YH, J. Taiwan Inst. Chem. Eng., 40, 13 (2009)
  12. Spolaore P, Joannis-Cassan C, Duran E, Isambert A, J. Biosci. Bioeng., 101(2), 87 (2006)
  13. Li Y, Horsman M, Wu N, Lan CQ, Dubois-Calero N, Biotechnol.Prog., 4, 815 (2008)
  14. Li Y, Wang B, Wu N, Li Y, Horsman M, Wang B, Wu N, Lan CQ, Appl. Microbiol. Biotechnol., 4, 629 (2008)
  15. Chisti Y, Biotech. Adv., 3, 294 (2007)
  16. Raja R, Hemaiswarya S, Kumar NA, Sridhar S, Rengasamy R, CRC. Cr. Rev. Microbiol., 2, 77 (2008)
  17. Huntley ME, Redalje DG, Mitig. Adapt. Strategies Glob., 4, 573 (2007)
  18. Bilanovic D, Andargatchew A, Kroeger T, Shelef G, Energy Conv. Manag., 50(2), 262 (2009)
  19. Natrah F, Yoso VFM, Shari VM, Yusoff FM, Shariff M, Abas F, Mariana NS, J. Appl. Phycol., 19, 711 (2007)
  20. Borowitzka MA, J. Appl. Phycol., 9, 393 (1997)
  21. Steen EJ, Kang Y, Bokinsky G, Hu Z, Schirmer A, McClure A, Keasling JD, Nature., 463, 559 (2010)
  22. Greenwell HC, Laurens LML, Shields RJ, Lovitt RW, Flynn KJ, Soc. Interface., 7, 703 (2010)
  23. Li X, Xu H, Wu Q, Biotechnol. Bioeng., 4, 764 (2007)
  24. Tsukahara K, Sawayama S, J. Jpn. Petrol. Inst., 5, 251 (2005)
  25. Torrey M, Inform., 7, 432 (2008)
  26. Renaud SM, Thinh LV, Lambrinidis G, Parry DL, Aquaculture., 1, 195 (2002)
  27. Pulz O, Gross W, Appl. Microbiol. Biotechnol., 6, 635 (2004)
  28. Chen F, Zhang Y, Guo S, Biotechnol. Lett., 5, 603 (1996)
  29. Carvalho AP, Malcata FX, Mar. Biotech., 7, 381 (2005)
  30. Caicedo JR, Van der Steen NP, Arce O, Gijzen HJ, Water Res., 15, 3829 (2000)
  31. Zeiler KG, Heacox DA, Toon ST, Kadam KL, Brown LM, Energy Convers. Manage., 36, 707 (1995)
  32. Liu W, Au DWT, Anderson DM, Lam PK, Wu RS, J. Exper. Mar. Biol. Ecol., 346, 76 (2007)
  33. Eriksen NT, Biotechnol. Lett., 30(9), 1525 (2008)
  34. Chiu SY, Kao CY, Tsai MT, Ong SC, Chen CH, Lin CS, Bioresour. Technol., 100(2), 833 (2009)
  35. Andrade MR, Costa JAV, Aquaculture., 264, 130 (2007)
  36. Illman AM, Scragg AH, Shales SW, Enzyme Microb. Technol., 27(8), 631 (2000)
  37. Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR, Biotechnol. Bioeng., 102(1), 100 (2009)
  38. Akdeniz N, Koziel JA, Ahn HK, Glanville TD, Crawford BP, Raman DR, Bioresour. Technol., 101(1), 71 (2010)
  39. Scragg AH, Illman AM, Carden A, Shales SW, Biomass Bioenerg., 1, 67 (2002)
  40. Gouveia L, Oliveira AC, J. Ind. Microbiol. Biot., 36, 269 (2009)
  41. Renaud SM, Thinh LV, Parry DL, Aquaculture., 170, 147 (1999)
  42. Hodaifa G, Mart nez ME, Sanchez S, Bioresour. Technol., 5, 1111 (2008)
  43. Bligh EG, Dayer WJ, Can. J. Biochem. Physiol., 37, 911 (1959)
  44. Huerlimann R, de Nys R, Heimann K, Biotechnol. Bioeng., 2, 245 (2010)
  45. Tran HL, Hong SJ, Lee CG, Biotechnol. Bioproc. Eng., 14, 187 (2009)
  46. Siaut M, Cuine S, Cagnon C, Fessler B, Nguyen M, Carrier P, Peltier G, BMC Biotechnol., 11, 7 (2011)
  47. Mendes RL, Reis AD, Palavra AF, Food Chem., 99, 57 (2006)
  48. Couto RM, Simoes PC, Reis A, Da Silva TL, Martins VH, Sanchez-Vicente Y, Eng. Life Sci., 2, 158 (2010)
  49. Harun R, Singh M, Forde GM, Danquah MK, Renew. Sust.Energy Rev., 14, 1037 (2010)
  50. Andrich G, Nesti U, Venturi F, Zinnai A, Fiorentini R, Eur. J.Lipid Sci. Technol., 107, 381 (2005)
  51. Andrich G, Zinnai A, Nesti U, Venturi F, Acta Aliment. Hung., 35, 195 (2006)
  52. Wijffels RH, Barbosa MJ, Science., 5993, 796 (2010)
  53. Dean AP, Sigee DC, Estrada B, Pittman JK, Bioresour. Technol., 101(12), 4499 (2010)
  54. Ryckebosch E, Muylaert K, Foubert I, J. Am. Oil Chem. Soc., 2, 189 (2012)
  55. Shen Y, Pei Z, Yuan W, Mao E, Int. J. Agric. Biol. Eng., 2, 51 (2009)
  56. Zhu M, Zhou PP, Yu LJ, Bioresour. Technol., 84(1), 93 (2002)
  57. Belarbi EH, Molina E, Chisti Y, Enzyme Microb. Technol., 26(7), 516 (2000)
  58. Lewis T, Nichols PD, McMeekin TA, J. Microbiol. Method., 43, 107 (2000)
  59. Pliquett U, Joshi RP, Sridhara V, Sridhara V, Schoenbach KH, Bioelectrochem., 2, 275 (2007)
  60. Soto C, Chamy R, Zuniga ME, Food Chem., 102, 834 (2007)
  61. Singh A, Nigam PS, Murphy JD, Bioresour. Technol., 1, 26 (2011)
  62. Sharma KK, Schuhmann H, Schenk PM, Energies., 5, 1532 (2012)
  63. de Morais MG, Costa JAV, Energy Convers. Manage., 7, 2169 (2007)
  64. Huang GH, Chen F, Wei D, Zhang XW, Chen G, Appl. Energy, 87(1), 38 (2010)
  65. Krohn BJ, McNeff CV, Yan B, Nowlan D, Bioresour. Technol., 1, 94 (2011)